Mining Of LincRNA In Wheat Under Fusarium Graminearum Infestation

Wheat(Triticum aestivum L.)is one of the most important food crops for humans,and the size of the hexaploid bread wheat genome is about 17 Gb.Wheat Head Blight caused by Fusarium graminearum is one of the catastrophic diseases in wheat production in China.In recent years,an increasing number of studies have shown that long intergenic non-coding RNAs(linc RNAs)play a role in disease resistance in plants.In this study,transcriptome data from 0h,24 h,48h and 72 h of F.graminearum infestation of wheat spikes were analyzed to screen and characterize the linc RNAs,and linc RNAs were analyzed for differential expression analysis,temporal expression analysis,cis-regulated target gene prediction and linc RNA-mi RNA-m RNA interaction analysis.Bioinformatics was used to study the resistance response of linc RNAs and their target genes to F.graminearum in wheat spikes.The main research results are as follows:1.LncRNAs can be classified into four types based on their relative position to protein-encoding genes in the genome: sense lnc RNA,antisense lnc RNA,intronic lnc RNA,and intergenic lnc RNA.Among these types,intergenic lnc RNA is the most prevalent,accounting for up to 90.8% of all lnc RNAs.Notably,the linc RNAs in wheat are evenly distributed across the chromosome and they are shorter and contain fewer exons than protein-coding genes.2.Bioinformatics analysis of linc RNAs associated with F.graminearum in wheat spikes: mining of transcriptome data from wheat inoculated with F.graminearum resulted in the screening of 1238 candidate linc RNAs and predicted that candidate linc RNAs may cis-regulate 1015 protein-coding genes.Functional enrichment of the encoded genes showed a significant enrichment in amino acid metabolism and cell membranes.Differential expression analysis of transcriptomic data from various time periods resulted in 548 differentially expressed linc RNAs.Based on the linc RNA-mi RNA-m RNA regulatory network,20 mature mi RNAs and their corresponding 1620 target genes were predicted in wheat.GO enrichment analysis of the target genes showed a significant enrichment for positive regulation of transcription via RNA polymerase II.An interaction network map was constructed for 11 linc RNAs and their 30 target genes based on the results of cis-regulation prediction.3.The detection of highly expressed differential genes by real-time fluorescence quantitative PCR revealed two pairs of linc RNAs with the same expression trend as their target genes.It was tentatively determined that they were involved in the regulation of disease resistance in wheat.In summary,this study identified important linc RNAs and their corresponding target genes in the process of wheat resistance to F.graminearum,and discussed the functions of the identified linc RNAs and their target genes.To further determine the specific functional mechanisms of linc RNAs in the process of wheat resistance to F.graminearum provide the theoretical and research basis.