| In this study,237 families and parents of the F8 generation recombinant inbred line population constructed by the high-yield medium gluten wheat variety Zhoumai 23 and the high-quality strong gluten variety Zhengmai 366 were used as experimental materials.The related indexes of wheat dough stretching characteristics and starch pasting characteristics were measured under five environments,and the genetic linkage map was constructed with the 55 K SNP chip of wheat.The main results were as follows:1.Construction of genetic linkage map: A high-density genetic map covering 21 chromosomes of wheat was constructed,which contained 2860 polymorphic markers with a total length of 19673.34 c M,the average distance between each marker was 6.88 c M,and there were 136 markers on each chromosome.2.QTL related to stretching parameters: A total of 25 QTL were detected for controlling wet gluten.QWGC.hau-2D.1 on 2D chromosome may be a new stable locus,which can be detected in E1,E2 and E4 environments,with an average Phenotypic Variation Explained of 4.31%.A total of 24 QTL controlling gluten index were detected,among which 5 were stable sites.A total of 8 QTL controlling settlement values were detected.A total of 116 QTL were detected for stretching parameters,which were located on 18 chromosomes and 13 stable sites.There were 28 QTL associated with Max extension resistance,and PVE ranged from 1.43% ~ 26.66%.QRmax.hau-1D.1 and QRmax.hau-4A.4 were detected in multiple environments.QRmax.hau-1D.1 were also detected in extension resistance and Max extension ratio,with an average contribution rate of 22.12%.QRmax.hau-1D.1was a stable QTL site,and its enhancing allele came from Zhoumai 23.The QRmax.hau-4A.4 site has a positive effect on the Max extension resistance,which has not been reported so far and may be a new site.There were 13 QTL related to extensibility,with Phenotypic Variation Explained ranging from 2.74%~23.87%.QE.hau-1B.1 and QE.hau-1D.2 were stable QTL detected in multiple environments.QE.hau-1D.2 could also be detected in stretching ratio,with an average contribution rate of 19.11%.The synergistic alleles were derived from Zhengmai 366,and the synergistic loci of QE.hau-1B.1 were derived from Zhengmai 366,with an average contribution rate of 4.68%.3.QTL related to RVA parameters: A total of 116 QTL were detected for RVA parameters,which were located on 21 chromosomes,and there were 11 stable sites.QPV.hau-4A.1,which controlled the peak viscosity,and QBD.hau-4A.1,which controlled the Breakdown,could be detected in more than two environments at the same time.The phenotypic variations of 2.55% ~ 24.23% and 11.5% ~ 48.30% were explained,respectively.The stable site QPV.hau-6A.1 detected on chromosome 6A for peak viscosity may be a new site,which was derived from Zhengmai 366,a high quality wheat variety with strong gluten,and had a significant synergistic effect on peak viscosity.It is related to the elasticity and toughness of noodles.In this study,we constructed a high density genetic linkage map and located important QTL loci related to wheat dough stretching characteristics and starch gelatinization characteristics,which laid a foundation for fine mapping and gene mining of wheat quality genes.It has important theoretical value and practical significance for breeding high-quality special wheat with coordinated quality by using molecular marker-assisted selection and molecular polymerization breeding. |
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