| Liver disease is one of the important causes of dog in clinical practice,which can be caused by various factors such as chemical drugs,viral infections,and trauma.In China,liver diseases account for 14.5% of the total number of dogs,and the incidence rate has increased year by year in recent years.For a long time,the diagnosis of liver diseases has been considered as a challenge,because the blood biomarkers of liver injury have certain limitations,while the live histopathology examination as the gold standard is more harmful to dogs.Mi RNA is a small molecule single stranded RNA that plays a role in regulating various life activities in organisms.It has specificity,stability,and sensitivity,is easy to detect,and has become a potential biomarker for various human diseases.At present,miRNA-122 has been found to have good specificity in acute liver injury in mice and humans,which has gained support from the US Food and Drug Administration as a potential biomarker for canine liver.However,there are currently few literature reports on the association of miRNA-122 in acute liver injury in dogs.Therefore,this experiment aims to establish a canine acute liver injury model,study the changes in the expression level of miRNA-122 during acute liver injury,discuss the diagnostic significance of miRNA-122,and provide theoretical basis for the development of biomarkers for canine acute liver injury.This experiment established an acute liver injury model by intramuscular injection of acetaminophen(APAP)solution.Twenty healthy Chinese pastoral dogs aged 1-2 years and weighing 5-8 kg were randomly divided into two groups: a control group(Con)and an Model group(M),with 10 dogs in each group.The M group was initially administered at a dose of800 mg/kg at 0 hours,followed by a second and third dose of 200 mg/kg at 9 and 24 hours.The Con group was intramuscularly injected with an equal volume of dimethyl sulfoxide solution at the same time.At 1,6,12,24,48,and 72 hours after the initial administration,clinical manifestations,CBC,and blood biochemical indicators of each dog were detected.The expression level of miRNA-122 was measured by RT-q PCR.At 72 hours,the hematocrit,thrombin time,partial thromboplastin activation time,prothrombin time,and fibrinogen level of the test dog were measured.Qualified test dogs underwent ultrasound-guided percutaneous liver puncture,and the liver status was checked by ultrasound,Collect an appropriate amount of liver tissue for histopathological examination and miRNA-122 expression level examination.The test results are as follows:The trend of white blood cell concentration in Group M showed a gradual increase,significantly higher than that in the Con group since 48 hours(P<0.05).The trend of red blood cell concentration showed an initial increase,reaching its peak at 48 hours,and then beginning to decrease.It was significantly higher in the Con group at 48 and 72 hours(P<0.05).The serum concentrations of alanine transaminase(ALT)and aspartate transaminase(AST)in group M were significantly higher than those in group Con from 24 h(P<0.05).At 72 hours,the histological examination results showed that the volume of liver cells in Group M increased and ballooning occurred. |
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