Analysis Of PgMYB Gene Family In Ginseng And Study On The Function Of PgMYB2

Background: In traditional medicine,ginseng(Panax ginseng)has long been used as a medicine in Asian countries to treat various diseases.Ginsenoside is the main active ingredient in the period,and has various pharmacological effects such as antioxidant,anti-inflammatory,and anti-cancer.With the gradual expansion of the market scale,ginsenosides have gradually become a scarce substance,which makes more and more people participate in ginseng research,and some key enzyme genes for ginsenoside synthesis have also been discovered one after another.Transcription factors,as proteins that can bind to gene promoter regions to regulate gene expression,have also received extensive attention.Although a large number of transcription factor functions have been clearly studied in model plants,there are few studies on the role of transcription factors in ginseng.Methods: The ginseng genome data in the public database was collected,and the ginseng MYB transcription factor family genes were mined by bioinformatics method for expression analysis.Using the previous research results of our group,we selected the PgMYB2 gene combined with the ginseng dammarediol synthase gene promoter,constructed an expression vector,and transferred it into the model plant tobacco to explore its expression in tobacco and its relationship with tobacco.related functional studies.Results: A total of 238 PgMYBs family genes were screened in the ginseng genome.GO annotation and enrichment analysis of 140 R2R3-PgMYBs genes indicated that the PgMYBs family played an important role in the growth and development of ginseng.Genes accounted for the highest proportion,in line with the description of the role of transcription factors in other studies.The PgMYB2 gene was successfully constructed into the overexpression vector pCAMBIA1301 S vector,and the vector was transferred into N.benthamiana to obtain positive plants.The expression levels of HMGR and HMGS genes were significantly up-regulated in tobacco plants,indicating that the transcription factor also has regulatory effects on secondary metabolic pathways in other plants.Conclusion: The PgMYBs gene family plays an important regulatory role in the growth and development of ginseng,and this family gene can be used to regulate the secondary metabolism of ginseng,and improve the production of ginsenosides to a new stage.In addition,PgMYB2,as one of them,not only has a very significant role in ginseng,but also can be expressed in other plants through transgenic means to regulate the metabolic functions of other plants,so as to achieve the purpose of obtaining high-quality germplasm resources.