Molecular Mechanism Of VrNAC In Response To Drought Stress In Mung Bean

With the increasing global drought problem,the research on the molecular mechanism of drought resistance of crops is becoming more and more important NAC transcription factors play an important role in plant stress growth,but the research on NAC transcription factors in mung bean is scarce.Related functional NAC transcription factors need to be explored Therefore,it is very important to explore the important NAC transcription factors in mung bean,which can not only enrich the research of NAC transcription factors in mung bean,but also lay a molecular foundation for cultivating drought-resistant mung bean materials In this study,two NAC transcription factors,VrNAC13 and VrNAC15,were cloned to predict their structures,and VrNAC15 was overexpressed in Arabidopsis thaliana to verify their functions.The results are as follows:(1)Cloning and sequence of VrNAC13 gene of mung bean The total length of VrNAC13 gene is 1068 bp,encoding 355 amino acids with NAM family conserved domain and transcriptional activation activity.The activation domain is located at the N-terminal of the sequence;Promoter VrNAC13 promoter contains promoter core elements and several cis-acting elements including gibberellin and abscisic acid under light stress;The results showed that VrNAC13 was involved in abiotic stress of drought and ABA and expressed specifically in leaves of mung bean;VrNAC13 was closely related to Arabidopsis AT1G60380.1 and Arabidopsis AT5G50820.1.(2)According to the structure of VrNAC15 gene,the total length of VrNAC15 gene is1014 bp,which encodes 337 amino acids.The conserved domain of NAM family is located in the nucleus,and the gene has transcriptional activation activity.The activation domain is located at the C end of the sequence;Promoter VrNAC15 promoter contains promoter core elements and cis-acting elements such as abscisic acid,methyl jasmonate,gibberellin photometabolism synthesis under stress;The dominant expression of VrNAC15 gene in leaves changed significantly after ABA and drought treatment;The relationship between VrNAC15 and AT1G01010.1 was observed by clustering.Recently,it was speculated that VrNAC15 might be involved in the defense response of mung bean,which involved ABA metabolism and water stress.(3)Phenotype identification of transgenic Arabidopsis thaliana showed that overexpression of VrNAC15 improved the tolerance and resistance of transgenic plants to drought stress Overexpression of VrNAC15 made Arabidopsis plants possess stronger antioxidant capacity and osmotic adjustment ability,and higher cell membrane structural integrity under drought stress to maintain the normal operation of intracellular physiological and biochemical metabolic reactions.In this study,the structure and function of VrNAC13 and VrNAC15 genes were elucidated by q RT-PCR,expression specificity of VrNAC13 and VrNAC15 genes and phenotype identification of transgenic Arabidopsis thaliana,which laid a foundation for drought-resistant molecular breeding of mung bean.