| Salvia miltiorrhiza Bunge is a perennial herb of Salvia in Lamiaceae.It is one of the bulk medicinal materials in China.It has the effects of promoting blood circulation,regulating menstruation,removing blood stasis and relieving pain.It is now widely used in medical drugs,health food,cosmetics and other fields.Sichuan Province is a real estate area of S.miltiorrhizais,and its S.miltiorrhizais has always been used as high-quality genuine medicinal materials at home and abroad.The survey found that in the real estate area of Sichuan Danshen Road,growers generally use brassinolide to improve the yield of Danshen.The study on the effect of Brassinolide on the yield and quality of S.miltiorrhizais is of great significance to maintain the authenticity and sustainable development of S.miltiorrhizais.Starting from the application amount and application period of brassinolide,this study explored the effects of Brassinolide on the growth and development,yield and effective components of S.miltiorrhizais,and analyzed the residue dynamics of brassinolide,in order to provide a theoretical basis for the standardized cultivation of S.miltiorrhizais,so as to ensure the quality and safety of S.miltiorrhizais.The main results are as follows:1.The effects of Brassinolide on the growth,development and yield of S.miltiorrhizais were evaluated by plant height,crown width,number of roots,root diameter,root length,root dry weight and root fresh weight.The results showed that0.05 mg/L and 0.1 mg/L brassinolide could significantly increase the plant height and crown width of S.miltiorrhizais and promote its growth and development at the seedling stage,flowering stage and root expansion stage;At the same time,brassinolide significantly increased the fresh weight and number of roots of S.miltiorrhizais and increased the yield of S.miltiorrhizais,with an increase rate of12.04% ～ 44.32%;SPSS analysis showed that the fresh weight of roots,the number of roots,plant height and crown width of S.miltiorrhizais had a significant impact on the yield.The degree of impact was as follows: fresh weight of roots > number of roots > crown width > plant height.2.The content of tanshinone(tanshinone IIA,tanshinone I and cryptotanshinone)and salvianolic acid B in S.miltiorrhizais were determined by HPLC to evaluate the effect of Brassinolide on its effective components.The results showed that the application of 0.05 mg/L brassinolide at seedling stage,flowering stage and root expansion stage significantly increased the content of tanshinone;The application of0.1 mg/L and 0.15 mg/L brassinolide at seedling stage and flowering stage significantly reduced the content of tanshinone;The application of brassinolide at seedling stage,flowering stage and root expansion stage of S.miltiorrhizais had no significant effect on the content of salvianolic acid B.3.Through continuous field sampling,the residue dynamics of brassinolide in Salvia miltiorrhiza plants and soil were analyzed,and the degradation curve of brassinolide was established.The results showed that the degradation of brassinolide in S.miltiorrhizais plant and soil accorded with its kinetic equation.The half-life in soil was 13.58 d and on plant was 9.62 d.4.Using principal component analysis,two principal components were extracted,and the cumulative contribution rate was 71.574%.Taking the number of root strips,root length,root diameter,root fresh weight,yield,tanshinone and salvianolic acid B content as evaluation indexes,and the comprehensive evaluation of membership function value,it is concluded that the application of 0.05 mg/L brassinolide at seedling stage has the most significant effect on the yield and effective components of S.miltiorrhizais. |
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