Integrative Analysis Of Transcriptome And Metabolome Reveals The Mechanism Of The Difference Of Flavonoid Content In Lithocarpus Polystachyus Rehd

Lithocarpus polystachyus Rehd has received much attention for its pharmacological activities such as inhibiting oxidation,and lowering blood glucose and blood pressure,and flavonoids are one of its main pharmacological components.They are widely distributed in plants,with various biological functions,such as the resistance to biotic and abiotic stresses,regulation of phytohormone activity,etc.Flavonoids are also one of the main active ingredients in many medicinal plants,with pharmacological activities like treating cancer,inflammation and cardiovascular diseases.Therefore,it is of great significance to understand the biosynthesis mechanism of flavonoids in L.polystachyus.In this study,differentially expressed genes and differentially accumulated metabolites in L.polystachyus were studied by integrating transcriptomics and metabolomics technologies.We confirmed the key genes involved in the flavonoid biosynthesis of L.polystachyus.The results of this comprehensive analysis showed that the key structural genes,LpPAL3,LpCHS1,LpCHS2,LpCHI2 and LpF3 H,in the flavonoid biosynthesis pathway of L.polystachyus had consistent expression patterns with their upstream and downstream metabolites,and there was a significant positive correlation between the key structural genes and their upstream and downstream metabolites.Compared to mature leaves,stems and young leaves are higher in the expression levels of key structural genes.LpMYB2,LpMYB20,LpMYB54 and LpMYB12,positively regulated the biosynthesis of flavones and flavanones.In addition,comprehensive analysis showed that abscisic acid and salicylic acid positively regulated the synthesis of flavonoid biosynthesis in L.polystachyus,and key candidate genes involved in the positive regulation of flavonoid biosynthesis were identified,including LpPP2C1,LpPP2C2,LpABF1,LpABF2 and LpNPR1.Figure 33;Table 12;Reference 96...