Effects Of Acetyl-thiazolidine-4-carboxylic Acid On Nutrient Uptake And Rhizosphere Soil Characteristics Of Maize

Maize is an important food crop in China,and it is also a crop that requires a large amount of fertilizer.The use of fertilizer has an important impact on the yield of maize.However,in actual production,there are often excessive use of chemical fertilizers,resulting in waste of resources and environmental pollution.In order to reduce the amount of fertilizer used and improve the utilization rate of fertilizers,this study investigated the effect of a new plant physiologically active substance,ATCA,as a fertilizer synergist on the nutrient uptake and utilization of maize and rhizosphere soil properties at different dosages,the main findings are as follows:(1)As a fertilizer synergist,exogenous addition of ATCA can increase the plant height of maize and promote nutrient absorption and dry matter accumulation in maize.The dry matter accumulation in the upper part of maize ground in WS and RS increased first and then decreased with the increase of ATCA dosage,and reached the maximum under the treatments of 10 mg/kg and 4 mg/kg,respectively.while the dry matter accumulation in the upper part of the maize ground in XS increased with the increase of ATCA dosage,reached the maximum at 20 mg/kg and increased by 15.8 %compared with the control.The 10 mg/kg treatment also increased 38.4 % N accumulation,23.2 % P accumulation and 29.5 % K accumulation in the upper part of maize in WS.Root application of ATCA can also increase the accumulation of P and K in the upper part of RS maize field,again with the 10 mg/kg treatment increasing P accumulation significantly and the 20 mg/kg treatment increasing K accumulation significantly,by 39.2 % and 40.2 %,respectively,compared to the control.Except for the 10 mg/kg treatment,other ATCA dosages showed inhibitory effects on the accumulation of P and K in the upper part of XS maize.Root application of ATCA also decreased the contents of Cu,Zn and Mn in the shoots of WS maize;increased the contents of Fe and Mn in the upper part of RS and XS maize,and increased with the increase of the dosage,and reached the maximum under the treatment of 20mg/kg.(2)Root application of ATCA increased the contents of AN and AP in the three tested soils.The 20 mg/kg treatment increased the content of AN in WS and RS,and the 4 mg/kg treatment increased the content of AN in XS.Compared with the control,10 mg/kg ATCA treatment can increase the content of AP in XS by 18.7% and the content of AK in WS by 4.8%,but the content of AK in XS decreased by each concentration of ATCA,which has no effect on improving the content of AK in RS.(3)Root application of ATCA decreased the urease activity in XS and RS,and increased the urease activity in WS,with the most pronounced increase in the 4 mg/kg treatment.Compared with the control,1 mg/kg ATCA treatment increased 3.57 %sucrase activity and 7.5 % CAT activity and decreased 3.0 % ACP activity in XS immediately after treatment 7 days.Different ATCA dosages reduced XS rhizosphere soil ACP and WS rhizosphere soil sucrase activities(4)The ACE index and Shannon index were used to characterize the bacterial community richness and diversity in the rhizosphere soil of maize.It was found that the bacterial richness was the highest in XS,followed by WS,and the lowest in RS.After ATCA treatment,the bacterial richness in WS was higher than that in the control treatment,and the bacterial richness in 4 mg/kg ATCA treatment was higher than that in 20 mg/kg treatment;the bacterial richness in XS was 20 mg/kg ATCA.The bacterial abundance in RS decreased with the increase of ATCA concentration,and there was no significant difference among the three soil treatments.NMDS analysis found that the addition of ATCA could significantly affect the bacterial community composition of the WS rhizosphere,increase the bacterial community richness and diversity,but had no significant effect on the changes in the bacterial community structure of RS and XS.The results of redundancy analysis found that soil available phosphorus content,ACP and urease activities had significant effects on environmental bacterial communities.(5)According to the results of this test,the optimum dosage of ATCA in different test soils was preliminary given.The best amount of hemicellulose was 10mg/kg in two test tidal soils;the best amount of hemicellulose was 20 mg/kg in test red soil,and adding ATCA can promote the absorption of nutrients by corn when the nutrient content is high.