| Since the total prohibition of adding antibiotics in feed,plant essential oils and tributyrin have been widely used in the production of broilers.Their main mode of action is to improve the structure of the intestinal flora and reduce the content of harmful bacteria in the intestinal flora.Promote the conversion and absorption of nutrients,thereby improving the immunity of animals,promoting animal growth,and further improving production performance.In order to evaluate the effects of plant essential oils and tributyrin in production,this study carried out an experiment on the effects of plant essential oils and tributyrin on ephedra chickens.The specific indicators include: production performance,including animal feed and meat ratio,increase Weight,survival rate,slaughter rate,etc.;intestinal flora,including the diversity of the flora,the types and quantities of beneficial and harmful bacteria,the composition of the intestinal flora;the microstructure of the intestine,including the integrity of the intestinal wall structure,The growth and development of small intestinal villi,the ratio of the length of intestinal villi to the depth of crypts;the expression of cytokines and intestinal wall tight binding proteins in the intestine;short-chain fatty acids in the intestine,such as acetic acid,propionic acid,butyric acid,and isobutyric acid.The content of valeric acid and isovaleric acid.The experimental results show that plant essential oils can increase broiler slaughter rate and reduce feed ratio;increase the ratio of Firmicutes and Bacteroides in the intestines,and increase the relative abundance of bifidobacteria;at the same time,plant essential oils can increase the length of broiler jejunum villi,but not The ratio of the length of intestinal villi to the depth of intestinal crypts(VH/CD)had no significant effect;the expression of IL-10 and Claudins-1 was increased but the difference was not significant.However,studies have found that plant essential oils can reduce the content of acetic acid in the jejunum,reduce the content of acetic acid,propionic acid,butyric acid and valeric acid in the cecum,and increase the content of isobutyric acid and isovaleric acid in the cecum.Tributyric acid can increase the slaughter rate of broilers and reduce the feed-to-meat ratio;adjust the structure of the intestinal flora,such as increasing the ratio of Firmicutes and Bacteroides in the intestinal flora,increase the relative abundance of bifidobacteria,and reduce E.coli Secondly,tributyrin can also increase the length of intestinal villi in broiler jejunum,but has no significant effect on the ratio of intestinal villi length to intestinal crypt depth(VH/CD);increase intestinal mucosal anti-inflammatory factor IL-10 and Claudins-1 expression,but the difference is not significant;in addition,tributyrin can also increase the content of acetic acid and butyric acid in the cecum,which is beneficial to the repair of the intestinal mucosa and maintains the acidic environment in the intestine.Promoting growth.According to the above indicators,from the macro to the micro,comprehensively evaluate the effects of plant essential oils and tributyrin on the production performance and intestinal health of broilers and establish a set of scientific and feasible evaluation methods.In the future,similar additive products can also be used.Use this set of evaluation methods for reference to systematically evaluate it.It is conducive to scientifically and systematically understand the promotion of plant essential oils and tributyrin on broiler chickens,and help breeding companies and farmers to better use additive products in the actual breeding process,so as to reduce the use of antibiotics and improve The purpose of production performance is to provide a little force for promoting the development of reducing and replacing resistance. |