Analysis Of Microbial Diversity In The Underground Of Lilium Brownii Var. Viridulum With Common Diseases And Different Cultivation Patterns

Lilium brownii var.viridulum is an economic crop with a long history of planting for medicine and food,and its scales have high nutritional value and health care effects.In recent years,diseases have frequently occurred in the process of planting Longya lily,especially the rot of underground bulbs and roots,resulting in reduced production and even plant withering and death,restricting the healthy development of the local Lilium brownii var.viridulum industry and affecting the quality and safety of lilies.In order to explore the relationship between the underground diseases of Lilium brownii var.viridulum and the diversity of microorganisms,this article examines the rhizosphere soil of healthy Lilium brownii var.viridulum,infected Lilium brownii var.viridulum,and root rot lily that have been planted in the field,washed with water and treated with surface sterilization.High-throughput sequencing of the scales and fleshy roots of Lilium brownii var.viridulum,and analysis of bacterial and fungal microbial diversity;the same method analyzed the open field cultivation mode(OFC)and plastic film mulching + one-time fertilization planting(PM&OTF)mode of planting soil The diversity of bacteria and fungi in the scales and fleshy roots of Lilium Longya and Lilium Longya has the following results:1.Bacterial microbial diversity analysis results: in rhizosphere soil and fleshy roots,Alpha diversity is: healthy lily> diseased lily> root rot lily(p<0.05);in scales,Alpha diversity is: root rot lily >Infected lily>Healthy lily.The results of beta analysis showed that the bacterial community structure of each material was not significantly different.Among all lilies,Proteobacteria,Actinobacteria and Firmicutes have relatively high relative abundance,accounting for more than 70% of the total bacterial sequence;and the abundance of Proteobacteria,Actinobacteria and Firmicutes The performance is: root rot lily> diseased lily>healthy lily(p<0.05),the actinomycete abundance performance is: healthy lily> diseased lily>root rot lily(p<0.05);at the genus level,each experimental material No obvious regularity was shown during the period;no pathogenic bacteria of the reported bacterial disease of Lilium brownii var.viridulum were detected.2.The results of fungal microbial Alpha diversity analysis: the distribution law of surface disinfection of lily scale diversity is as follows: root-rot lily>infected lily>healthy lily(p<0.05);rhizosphere soil,fleshy roots and lily scales washed with water,Healthy lily>infected lily>root rot lily(p<0.05).The results of principal component analysis of fungal community structure among all materials were not significantly different.At the phylum level,the relative abundance of the three phylum Ascomycota,Basidiomycota and Mortierellomycota of all materials is relatively high,accounting for more than 85% of the total sequence of fungi;and Ascomycota The phylum abundance performance is: root rot lily> diseased lily> healthy lily(p<0.05),Mortierella abundance performance is: healthy lily> diseased lily> root rot lily(p<0.05);at the genus level,All Lilium brownii var.viridulum scales and fleshy roots of Lilium brownii var.viridulum showed high abundance of Fusarium,which was specifically expressed as: Root-rot lily>Infected lily>Healthy lily(p<0.05),while other genera were not Shows a significant difference.Comparing the relative abundance of Fusarium in different parts of the infected and root rot of Lilium brownii var.viridulum,the rule is: rhizosphere soil> fleshy roots> scales(p<0.05),which is consistent with the severity of underground diseases of Lilium brownii var.viridulum;The soil,lily scales and fleshy roots can all be spreaders of the pathogen Fusarium.3.Comparing the soil of the mulching film + one-time fertilization planting(PM&OTF)mode and the traditional open field cultivation(OFC)mode,the soil fungal community richness of the PM&OTF mode is lower than that of the OFC mode,but there is no significant difference in the community diversity(p>0.05);There was no significant difference in the bacterial richness and diversity of the soil between the two planting modes(p>0.05).According to the principal component analysis,the soil microbial community structure of the PM&OTF model is closer to the blank land than the OFC model.In the analysis of soil fungi and bacterial community composition in the planting land,there was no significant difference in microbial diversity between the two planting modes at the phylum level(p>0.05);at the genus level,it showed that Fusarium was relatively abundant in the PM&OTF model.The degree is significantly lower than that of OFC mode(p<0.05).Analysis of the community composition of the lily scales and fleshy roots of the two planting modes showed that the abundance of Fusarium in the PM&OTF model of Aralia lily was significantly lower than that of the OFC model(p<0.05),and the PM&OTF model of Aralia lily scales and fleshy roots There is a higher diversity of microorganisms.The analysis results show that the PM&OTF model has the potential to control and alleviate the occurrence of diseases of Lilium brownii var.viridulum,which is consistent with the actual production performance.