| Crassostrea gigas is a shellfish of the genus Crassostrea in the Oyster family.C.gigas camp sessile life,larvae after a period of planktonic life,metamorphosis development attached to the object.Because of its social characteristics,the density of seedlings is too high,and the shell is generally irregular after being squeezed,which can form oyster reefs.The shell of C.gigas is formed by mantle biomineralization,but the shell development on the left and right sides is asymmetrical.The left shell of C.gigas is larger and plays a fixed role.Once it is fixed on the object,it cannot move all its life.The right shell is smaller and has the ability to move,which can freely close up and down to filter and feed on organisms in seawater.At present,the mechanism of larval attachment metamorphosis of C.gigas has been thoroughly studied.However,there are few reports on whether the adult C.gigas has refixation ability and the specific molecular mechanism.Through studies,scholars found that the detached C.gigas had a certain ability of reattachment,but they did not explore the substances that could reattach.We can artificially remove C.gigas from the attachment base for refixation,record its refixation process,study the potential of refixation,detect the substances that make it refixation,and infer the refixation mechanism.Therefore,this study explored and verified the refixation potential of C.gigas.This study with long oyster as the research object,from the oyster breeding raft frame on purchasing the same batch of oysters,oysters are attached to the object will be stripped,adhered to the removal of the oyster shell,remove the left shell surface scales with the method of artificial polishing burnish of left shell to reveal the prism layer,make its left shell damaged thinning,stimulating growth of oyster shell membrane repair.The polished oysters were fixed on cement bricks,rubber,ground glass and other new attachments,and placed in the cofferdam pond in the sea area.The growth,repair and refixation of oysters were observed and recorded.The experimental results showed that after the left shell was damaged,a new transparent shell film would grow at the edge of the shell.At this time,although the shell film would closely adhere to the surface of the attachment for growth and repair,and presented a "symmetric repair",it could not be firmly attached again.In order to further study the molecular mechanism of shell repair and growth after left shell injury,we analyzed the capsid transcriptome after left shell injury,and found that the differentially up-regulated genes in the left capsid were significantly enriched in some signal transduction and signal regulation functions at 1,5,9,13,17 and 21 days after left shell injury.After injury,down-regulated genes were significantly enriched in response to temperature and abiotic stimuli.Through the gene expression,we also found that adhesion-related genes such as Cgfmp,Cg Tyr and EGF-P1 were down-regulated after shell damage,which may lead to the failure of shell resettlement.To understand the potential of refixation of C.gigas by studying the repair and refixation of damaged left shell.It has laid a foundation for the mechanism research of biological mineralization and biological attachment,and has potential application value for the optimization of oyster cultivation methods,the artificial restoration of oyster reefs,and the prevention and control of oyster fouling. |
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