Identification And Expression Profile Analysis Of Odorant Receptors In Clostera Restitura Walker

Odorant receptors are transmembrane proteins located on the dendrites of sensory neurons,which determine the selectivity and specificity of neurons to odor molecules.Pheromone receptors(PRs)are a special class of receptors involved in sensory pheromones.Therefore,PR gene is the key factor determining the accurate mate recognition of male moths.In this study,we identified OR genes from transcriptome data and filtrated the OR genes that were highly expressed in male antennae or co-expressed in female glands of Clostera restitura,a disastrous defoliator of Poplus,phylogenetic analysis was carried out,and q RT-PCR was used to analyze their expression levels in different genders and tissues.The main results are as follows:A total of 89324 Unigenes were identified by transcriptional sequencing of the glands and male genitalia of C.restitura,the average length of Unigenes was 760.28 bp.64 putative odorant receptors(ORs)genes were identified from transcriptome data,including 1 odorant receptor coreceptor(Orco),4putative pheromone receptors(PRs)and 59 common odorant receptors.q RT-PCR was applied to determine the expression profile of ORs with high expression level,results showed that Orco gene was equally expressed in the antennae of male and female,and the other five OR genes were significantly highly expressed in male antennae.As a result,Cres OR42、56 and Cres＿g＿OR5 were determined to be putative PRs.These researches may lay the foundation for pheromone recognition mechanisms.