Evaluation And Analysis Of Clinical Effect Of Kushen Cangzhu Granules On Broiler Colibacillosis

Chicken colibacillosis is a common bacterial infectious disease caused by pathogenic Escherichia coli,which brings huge economic losses to the poultry industry and poses a potential threat to human life safety.Control is not only related to the development of chicken industry,but also has important public health significance.However,due to the emergence of drug resistance of Escherichia coli,the prevention and treatment of chicken colibacillosis has become difficult,and the research on the use of non-resistant drugs to control chicken colibacillosis has attracted more and more attention.Kushen Cangzhu oral liquid has been used to treat chicken colibacillosis,but the stability of the oral liquid is not ideal.The granules after the improvement of the preparation process have high content and activity of active ingredients,and the effect is more stable.However,Kushen Cangzhu granules have not yet been used in clinical trials.Therefore,the purpose of this experiment was to study the clinical application effect of Kushen Cangzhu granules on Escherichia coli-infected broilers,and to explore the effects of the drug on the intestinal mucosal mechanical barrier,liver cholesterol metabolism,and intestinal and intestinal metabolism of Escherichia coli-infected broilers.The effects of liver antioxidant enzymes and detoxification enzymes activities provide theoretical guidance for the clinical application of Kushen Cangzhu granules during broiler breeding and the prevention and treatment of chicken colibacillosis in actual production.The contents and results of this study are as follows:Forty-eight 21-day-old white-feathered broilers were randomly divided into blank control group,model control group,pretreatment group,Kushen Cangzhu granules low-dose group,middle-dose group and high-dose group,with 8 in each group.The pretreatment group was added Kushen Cangzhu granules(3.6 g/L)in drinking water,and 0.7 m L of pathogenic Escherichia coli bacteria solution(8.37×10~8 CFU/m L)was injected intramuscularly in the chest after 7 days of free drinking;The treatment groups with different doses were injected with Escherichia coli bacteria solution first,and after24 h of inoculation,the treatment groups with different doses of drugs were then added with different doses of Kushen Cangzhu granules(low-dose group:3.6 g/L,medium-dose group:5.5 g/L,high-dose group:7.3 g/L),drink freely for 7 days.At the end of the experiment,all experimental broilers were sacrificed,blood was collected,tissue samples were collected,and body weight and the weight of each organ were recorded;the morphological changes of liver and small intestine were observed by paraffin section technology;changes in blood lipids and liver injury-related indexes in serum were detected;total cholesterol(TCH),triglyceride(TG),high and low density lipoprotein cholesterol(HDL-C)(LDL-C)content and glutathione peroxidase(GSH-PX),glutathione S-transferase(GST)enzyme activity changes;RT-q PCR was used to detect the changes of intestinal mucosal mechanical barrier,liver cholesterol metabolism,small intestine and liver antioxidant enzymes and metabolic enzyme-related genes.1.Compared with the model control group,the low-dose group significantly decreased the liver index,jejunum index,and serum alkaline phosphatase content of Escherichia coli broilers;the low-and high-dose groups significantly decreased the serum TG content(P<0.05),and Kushen Cangzhu granules reduced the serum TG content significantly(P<0.05).Inflammatory cell infiltration in liver tissue significantly increased duodenal chorionic-to-crypto ratio,pretreatment group significantly increased jejunal chorionic-to-crypto ratio,pretreatment group and low-dose group significantly increased ileal chorionic-to-crypto ratio(P<0.05),pretreatment group,medium and high dose groups significantly increased liver LDL-C/HDL-C ratio(P<0.05),high dose group significantly decreased aspartate aminotransferase gene expression level(P<0.05),and pretreatment group significantly increased 3-Hydroxy-3-methylglutaryl-Co A reductase(Hmgcr)m RNA level(P<0.05),the low and middle dose groups significantly or extremely significantly increased Hmgcr,Sterol regulation Sterol-regulatory element binding proteins 2(Srebp2)m RNA levels(P<0.05,P<0.01),the middle dose group significantly increased the Scap m RNA level(P<0.01),and each dose group significantly or extremely significantly increased Cyp7a1 m RNA levels(P<0.05,P<0.01).The low-dose group significantly decreased the ileal transmembrane protein Occludin m RNA level(P<0.05).2.Compared with the model control group,the GSH-PX enzyme activity in the jejunum was significantly or extremely significantly decreased in each dose group(P<0.05,P<0.01),the GST enzyme activity was significantly increased in the middle dose group(P<0.05),and the high dose group was significantly Increased duodenal and jejunal catalase(Cat)m RNA levels(P<0.05),pretreatment group significantly increased duodenal Cyp1a1 m RNA level(P<0.05),high-dose group significantly increased duodenal Intestinal Cyp2a6 m RNA levels(P<0.05),pretreatment,low and high dose groups significantly or extremely significantly increased duodenal Gstm2m RNA levels(P<0.05,P<0.01),but the low dose group significantly decreased duodenal Gstm2 m RNA levels(P<0.05,P<0.01)Intestinal Gsta4 m RNA level(P<0.05);the low and middle dose groups significantly increased the jejunal Gsta2 m RNA level(P<0.05),the high dose group significantly increased the Gstm2 m RNA level(P<0.05),and the high dose group significantly increased the duodenum The expression level of Jnk1 m RNA level(P<0.05),the middle-dose group significantly increased the jejunal P38,Jnk1 m RNA level(P<0.05);the high-dose group significantly increased the ileal nuclear factor E2 related factor 2(Nrf2),Kelch-like epichlorohydrin-related protein 1 m RNA level(P<0.05).3.Compared with the model control group,the pretreatment group,medium and high dose groups significantly decreased the expression level of hepatic superoxide dismutase 2(Sod2)m RNA(P<0.05),and each drug dose group significantly or extremely significantly increased the liver GST enzyme activity(P<0.05,P<0.01),Kushen Cangzhu granules significantly or extremely significantly increased hepatic Cyp2a6 and Gsta3 m RNA levels(P<0.05,P<0.01),and pretreatment group significantly increased hepatic protein kinase(Erk),C-Jun N-terminal kinase 1(Jnk1)m RNA level(P<0.05),the high-dose group significantly increased Jnk2 m RNA level(P<0.05),pretreatment the m RNA level of Nrf2 was significantly increased in group,low and medium dose groups(P<0.05).In conclusion,Kushen Cangzhu granules can alleviate the decrease of intestinal digestion and absorption function,inflammatory cell infiltration and abnormal cholesterol metabolism in liver tissue caused by Escherichia coli infection in broilers,but have no significant effect on the mechanical barrier function of small intestinal mucosa;The transcription level of JNK can inhibit the activity of antioxidant enzymes and promote the activity of detoxification enzymes,while in the liver,it can play the role of promoting antioxidant enzymes and detoxification enzymes by significantly increasing the transcription level of ERK/JNK-Nrf2.