Prediction Of The Potential Distribution Of Two Pests Of Pseudaulacaspis And Analysis Of The SSR Information Of Transcriptome Of Pseudaulacaspis Cockerelli (Cooley)

Pseudaulacaspis belongs to the order Hemiptera and Diapididae.The pests of this genus have a wide range of feeding habits.They use piercing and sucking mouthparts to feed on the sap of host plants and endanger agricultural and forestry production.In order to explore the potential distribution range of the two species of pests,this paper combines the existing distribution sites and current and future environmental and climate data,and uses Max Ent software to predict their potential distribution areas and their diffusion dynamics under climate change;in addition,in order to understand the occurrence of Pseudaulacaspis cockerelli,this article uses high-throughput sequencing technology for the first time to analyze the transcriptome of P.cockerelli.The study obtained the current potential distribution range of the two pests and the spread dynamics under future conditions,which provided a reference basis for the formulation of their control strategies;at the same time,the transcriptome database of P.cockerelli was established,and the microsatellite(SSR)was developed,Simple Sequence Repeat Polymorphisms molecular markers,which is conducive to fundamentally understand the adaptation mechanism and disaster mechanism of P.cockerelli,so as to provide a theoretical basis for the control and management of P.cockerelli regional disaster.The main findings of this study are as follows:1.Prediction of the potential distribution area of Pseudaulacaspi pentagona.The prediction results show that Asia,Europe,South America and North America are highly suitable areas for this pest,and large-scale outbreaks of P.pentagona in the highly suitable areas should be prevented.Under future climatic conditions,global warming may lead to further expansion of the highly suitable areas and suitable areas for P.pentagona in the high latitudes of the north.The mulberry scales will shift northward,and P.pentagona will spread in East Asia,Japan,Europe,and North America.Growth,the area of suitable areas in South America has shrunk,resulting in a steady increase in the area of suitable areas and continuous growth.The dominant climatic factors affecting the distribution of P.pentagona are: BIO2,BIO3,BIO8,and BIO15,among which BIO3 is the most important factor affecting the potential distribution of P.pentagona,with a contribution rate of 48.6%.2.Prediction of the potential distribution of P.cockerelli.The prediction results indicate that East Asia,Europe and the eastern part of the Americas are highly suitable areas for this pest.Under future climatic conditions,the suitable areas for North America,South America,Africa,and Australia will shrink,and the suitable areas for Europe,East Asia,and Japan will increase significantly,especially in Japan,where the growth rate of highly suitable areas is twice as large as the current one.To prevent the large-scale outbreak of P.cockerelli.Under future climatic conditions,the dominant climatic factors affecting the distribution of P.cockerelli are: BIO2,BIO3,BIO8,and BIO15.BIO3 is the most important factor influencing the potential distribution of P.cockerelli,with a contribution rate of 38.8%,followed by BIO2,with a contribution value of 33.9%.3.Characteristic analysis of SSR locus in the transcriptome of P.cockerelli.In this study,for the first time,transcriptome sequencing and analysis of the whole worm of P.cockerelli were carried out,and the transcriptome database of P.cockerelli was established.Through the analysis and research of transcriptome data,70566 unigenes were assembled and spliced,which were compared to seven public databases of Nr,Nt,KO,Swiss Prot,Pfam,GO and KOG,with 40093,5953,21465,33840,39113,39113,and 22666 unigenes were annotated to the above public database.A total of 46,882 unigenes were successfully annotated in at least 1 database,and the annotation success rate reached 66.43%.On the basis of transcriptome sequencing,the MISA software search method was used to search,screen and analyze the SSR sites in the transcriptome of P.cockerelli.The results showed that there were 56857 SSR sites,and the distribution frequency of SSR was about 80.99%.,On average,there will be 1 SSR for every 2340.78 kb.The repetitive types of SSR distribution sites mainly include single-nucleotide,di-nucleotide and tri-nucleotide repetitive types,accounting for 96.07% of the total SSR.50 pairs of designed SSR primers were randomly selected to perform PCR amplification of SSR sites on 8 samples of adult worms from different origins of P.cockerelli,15 pairs of primers can amplify a single and stable specific target band with an effective amplification efficiency of 30%.