Study On Pre-digestion Of Piglet’s Diet With Compound Enzyme

The research and application of non-antibiotic diets is currently one of the hot spots in pig production.The feed enzymatic pre-digestion technology can decompose complex macromolecular substances into beneficial small molecules and reduce anti-nutritional factors.It could promote the digestion and absorption of nutrients,and maintain piglet intestines.The advantages of healthy development of the tract are a new trend in the development of non-resistant diets,but the research on related pre-digestion technologies is still in the exploratory stage.Firstly,this study selected 11 enzyme preparations,including protease,amylase,phytase,non-starch polysaccharide enzymes(cellulase,pectinase,xylanase,β-glucanase and α-galactosidase),glucose oxidation enzyme,ferulic acid esterase and arabinofuranosidase.According to the physical and chemical properties of the enzyme,this experiment explores the effect of enzyme action temperature and time on the pre-digested piglet diet,selects the best combination method,and determines the enzymatic predigestion.Secondly,the use of scanning electron microscopy and laser confocal combined with quantitative technology to assess the structural changes of the enzymatic feed.Finally,the use of in vitro digestion system to evaluate the dry matter digestibility of the pre-digested feed and the release of reducing sugars.The results of the study are as follows:1.Protease and phytase.After pre-digestion in a buffer of pH=3,the amino acid content,small peptide molecules and phosphorus content increased significantly(P<0.05)when exposed to 50 ℃ for 0.5 h.Therefore,it can be determined that at pH=3,50 ℃,add protease and phytase for 0.5 h.Amylase and NSP enzymes are added to the diet.The feed is reacted in a buffer with pH=5.After 1 h of pre-digestion,the reducing sugar at 50 ℃ was the highest(P<0.01)detected by DNS method,and the anti-nutritional factors at 50 ℃ were the least(P<0.01)detected by ELISA.So,it can be determined that the pre-digestion condition for adding amylase and NSP enzyme is 50 ℃,enzymatic hydrolysis for 1 h.2.In order to determine the hydrolysis effect of xylan,this experiment analyzed the synergistic effect of the xylan backbone enzyme and its branched chain enzymes.The results showed that ferulic acid esterase and arabinofuranosidase were added first,and then xylanase was added to hydrolyze the feed.The release of reducing sugar increased by 56%.Therefore,ferulic acid esterase and arabinofuranosidase were added for enzymatic hydrolysis for 1 h.In addition,adding glucose oxidase produces a gluconic acid content of 1.32μg/m L,and reduces the pH of the feed.3.Scanning electron microscopy and Rhodamine B staining combined with laser confocal photography showed that the cell wall of the feed after the compound enzyme predigestion was severely damaged and the complete structure was destroyed.At the same time,the test feed for E.coli and Salmonella isolated from diarrhea pigs was selected in this experiment.The results showed that the feed after enzymolysis and predigestion can inhibit the growth of E.coli and Salmonella.4.Vitro simulated digestion testing found that pre-digested feed vitro dry matter digestibility was significantly higher than that of the control group(P<0.01),reducing sugar release is also very significantly higher than that of the control group(P<0.01).In summary,this study determined the optimal conditions for the pre-digestion of piglet dietary compound enzyme preparations,identified its effects,and improved the pre-digestion technology system,providing a reference for the development of non-resistant diets for weaned piglets.The application and promotion of alleviating piglet weaning stress provides theoretical support.