Phenotype Analysis And Rapid Propagation Research Of Some Plants Of Rosa L.

The Rosa L.belongs to the Rosaceae family.There are about 200 species in the genus,which are widely distributed in the temperate zone of the northern hemisphere to subtropical regions.The Rosa L.cultivation has a long history,is one of the world’s famous ornamental plants,is also a common garden plant.After a long period of growth and cultivation,the plants of the Rosa L.are relatively complicated,and the relationship between the relatives within the genus is complicated.People often confuse the‘rose’in the genus Rosa.These all bring difficulties to the research problems of Rosa L.variety resources investigation,variety classification and identification,phylogeny,etc.At the same time,it also limits the genetic improvement process of the Rosa L.and their application in agricultural extension.In this paper,we studied the classification and kinship of some plants about Rosa L.from all over the world.At the same time,we selected some of them for cutting and tissue culture experiments to provide reference for subsequent agricultural promotion.The main results are as follows:1.Morphological observationThe eight morphological indicators of 80 varieties of Rosa L.were investigated and statistically studied,and the results showed that the morphological differences of stem and leaf spines among the varieties were large,and the morphological performance was rich and diverse.There are obvious differences in the shape of the prickles between the different species.The four prickles（straight,diagonal,curved,and hook）all exist.There are also obvious differences in the size of the leaves and the number of small leaves in the compound leaves.2.Principal component analysis of phenotypic traitsPrincipal component analysis of the stem and leaf spines of 80 varieties of the Rosa L.:According to the total variance explanation table,among the traits of roots,stems and leaves,three components dominate,and the impact of the total contribution rate of the three components reaches 61.609%;Among them,the first principal component includes three indicators,namely:internode length,tip leaflet length and leaflet number in compound leaves;These morphological traits play a very important role in population variation,and can be applied to the main morphological traits of their varieties.3.Morphological cluster analysisUsing the average connection method between clusters for systematic cluster analysis,at five European distances,80 rose species were divided into 3 groups.The basis of clustering is mainly based on geographical location.However,due to continuous cross breeding of the Rosa L.,the kinship relationship is more complicated.It is difficult to separate all varieties through only some traits,and further investigation is still required.4.Cutting rapid propagationThrough cutting propagation technology,50 varieties of the Rosa L.were rapidly propagated,and it was found that each variety was affected by genetic characteristics,and the rooting rate was very different.Among them,it can be seen from the rooting survival rate from high to bottom,the R.maximowicziana Regel has the highest rooting survival rate,which can reach 82.22%,The survival rate of the R.hybrida‘Nei Xiang’is second,up to 80.56%.Several rose varieties,such as R.rugosa‘Mici’,R.rugosa‘Jingyou2’and R.rugosa‘Jingyou1’have lower rooting survival rates,all of which are less than 5%.There are 10 hybrid rose varieties R.hybrida‘Abraham Darby’,R.hybrida‘Sheherazad’,R.hybrida‘Eclair’,R.hybrida‘The Poet’s Wife’,R.hybrida‘swallow’,R.hybrida‘Glamis Castle’,R.hybrida‘Blue Eden’,R.hybrida‘Accademia’,R.hybrida‘Libellula’,R.hybrida‘Nahéma’could not take root and survive in this experimental treatment,it may be necessary to continue to explore suitable rooting propagation methods.5.Tissue cultureA preliminary study on the aseptic systems of the four rose species R.gallica var.‘Dian Hong’,R.rugosa cv.‘Mici’,R.rugosa‘Mici’and R.damascena found that:（1）In the primary culture,NAA had a significant effect on the induction of axillary buds of three varieties except R.rugosa‘Mici’::The optimal concentrations of R.gallica var.‘Dian Hong’and R.damascena are 0.05mg·L^-l,and R.rugosa cv.‘Mici is 0.10mg·L^-l and 0.20mg·L^-l.（2）At the same time,in the primary culture,6-BA had a significant effect on the induction of axillary buds of three varieties except R.rugosa cv.‘Mici’:R.gallica var.‘Dian Hong’is 2.0mg·L^-l;R.rugosa‘Mici’and R.damascena are 1.5mg·L^-l;（3）In the proliferation culture,only R.gallica var.‘Dian Hong’was tested,and it was found that the optimal concentration of 6-BA is 2.0 mg·L^-l,and NAA may inhibit its value-added.（4）In the rooting culture,continuing the preliminary research on R.gallica var.‘Dian Hong’found:the optimal concentration of AC is 1.0g·L^-l;the optimal concentration gradient of NAA is 0.05mg·L^-l,and there is no significant difference between the three concentration gradients.