| Microbial fermentation feed is the use of specific microbial strains to play the role of pre-digestion,decomposition of macromolecules,degradation of anti-nutrient factors,the production of livestock can be more easily to eat,digestion,absorption of metabolites.Fermented feed can not only improve the nutritional value of feed,but also play a role in regulating intestinal flora and maintaining intestinal health.At present,the strains of microbial fermentation feed are commercial yeast,lactobacillus,bacillus subtilis and other exogenous beneficial bacteria,which are mixed or fermented by a single strain.Although commercial strains have a strong digestive capacity,they are generally difficult to colonize the intestines of livestock and poultry.In recent years,there are complex microbial flora in the intestinal tract of livestock and poultry,in which the proportion of beneficial bacteria is high,which can well maintain the intestinal health,promote the digestion and absorption of nutrients and the growth of livestock and poultry.However,there has been no report on the use of intestinal endogenous lactic acid bacteria to ferment feed.In order to study the fermentation feed of intestinal endogenous lactobacillus,this paper isolated and cultured intestinal probiotic lactobacillus by collecting intestinal microorganisms from different pig and chicken sources.The growth curve,pH value,acid production capacity and metabolite synthesis capacity of the obtained lactobacillus were screened.Then the probiotic effect of lactobacillus was evaluated by intragastric test.The contents of crude protein,crude fiber,protein degradation state and metabolic intermediates were evaluated by solid state fermentation.Meanwhile,the composition of some endogenous probiotic flora was analyzed by 16 S sequencing.On this basis,several lactobacilli were selected to prepare full-price fermentation feed for broilers,and the changes of growth performance and meat quality of broilers were observed.The results showed that the growth curve of endogenous lactobacillus was basically balanced after 3 days of culture,and the pH value was generally below 3.8.Among them,F16 showed higher acid production capacity,with the highest content of lactic acid(85 mmol/L),followed by a small amount of succinic acid(6.3 mg/mL)and lketoglutaric acid(220 g/mL).Co-irrigation of all strains and salmonella bacteria could reverse the anorexia and weight loss caused by salmonella bacteria to different degrees(P<0.05).After fermented soybean meal with different intestinal lactobacillus,large proteins of 50-70 k and small proteins of 10-15 k can be decomposed specifically,and some medium proteins of 25-40 k can also be decomposed.Metagenomic results showed that the cultivated intestinal bacteria were mainly composed of lactobacillus and bacillus,of which the lactobacillus in F04 was more than 90%.At the species level,most of the bacteria were mainly composed of lactobacillus and lactobacillus.The results of the fermentation of fullvalue feed showed that the fermentation of full-value feed could significantly increase the weight gain of broilers,promote their intake,improve the utilization rate of feed,and improve the hardness of pectoral-leg muscle in terms of meat quality.It also promotes the flavor of chicken to a certain extent,and can improve the taste and odor of hamstring soup/meat with obvious effects.Among them,F17 can mainly improve the special flavor and umami taste of hamstring.In summary,it was found that the isolated and cultured endogenous lactobacillus could be used to ferment feed to improve the production performance and meat quality of broilers.The results of this study have reference value for the study of intestinal bacteria and probiotics fermentation feeds of different livestock and poultry. |
PDF Full Text Request