Light Regulates HsfA1d Through CRY1 To Improve Plant Heat Resistance

Light,as an important environmental signal,on the one hand provides energy for plant photosynthesis,and on the other hand,it can regulate the growth and development of plants.Higher plants have evolved a sophisticated light signal transduction system that regulates plant growth and development.In nature,there is an inseparable relationship between light and temperature,and temperature increases with the increase of light intensity.High temperature is a common abiotic stress.The increase in temperature induces heat shock response in plants and improves their heat tolerance.Heat shock can induce plants to produce heat shock proteins,and heat shock proteins can act as molecular chaperones to maintain normal functions of other proteins at high temperatures.Light and temperature are very important for plants.There are few studies on the relationship between light signal transduction and high temperature resistance in plants.In this study,Arabidopsis was used as experimental material,and it was found that the blue light receptor CRY1 was involved in photoinduced heat shock response to enhance its heat resistance.The main research results are as follows :(1)By comparing the survival ability of wheat,rape and Arabidopsis after photo and thermal pretreatment at lethal high temperature,it was determined that light could replace heat and participate in plant heat resistance.Semi-quantitative and RNA-Seq sequencing were used to analyze the gene expression profiles of wild-type Arabidopsis under light and heat treatment,which proved the indivisible relationship between light and heat.(2)By analyzing the survival ability of wild-type Arabidopsis thaliana under lethal high temperature after different light quality treatments,and the survival ability of different light receptor mutants under lethal high temperature after light pretreatment and thermal pretreatment,it was found that CRY1 is a key regulatory factor involved in blue light-induced heat shock response.Subsequently,AtHsf A1 d gene was transformed into Arabidopsis under Athsf A1 s QK background,and the heat shock response ability was restored,indicating that AtHsf A1 d plays an important role in light-induced heat tolerance.Since AtCRY1 and AtHsf A1 d are both involved in photoinduced heat tolerance,the interaction between AtCRY1 and AtHsf A1 d was verified by yeast two-hybrid and in vivo Co-IP,and it was verified by Western blotting that light can regulate the nuclear localization of AtHsf A1 d to participate in heat shock response.(3)By transferring AtCRY1 into yeast cells,it was found that AtCRY1 could weaken the deposition of unfolded proteins in yeast under heat shock conditions.Compared with the heat resistance of wild-type yeast,AtCRY1 could enhance the heat resistance of yeast.Atthe same time,the wheat and canola after blue light treatment were exposed to 50°C,and the survival rate was significantly improved,indicating that blue light signal played an important role in improving crop heat resistance.In summary,we found that light signal could induce heat shock response,and blue light receptor AtCRY1 participated in plant heat shock response by regulating AtHsf A1 d,thereby improving plant heat tolerance.In this study,the light signal and heat signal pathways are linked to provide a theoretical basis for the use of light receptors for crop high temperature resistance improvement.