Analysis Of Mechanism Of Strawberry Fruit Ripening Regulated By AREB/ABF/ABI5 Gene Family

Fruit development and maturity has always been a hotspot and difficulty in the research of fruit trees.A deep understanding of the molecular mechanism of fruit development and maturity will not only help to improve the yield and quality of fruits,promote efficient storage,transportation and freshness,but also promote the structural adjustment of the fruit industry and development.Studies have shown that abscisic acid(ABA)not only plays a key role in plant development and adaptation to environmental stress,but also plays an important role in regulating fruit ripening.Members of the ABA response element binding protein / ABRE binding factor and ABA INSENSITIVE 5(ABF / ABI5 / DPBF)gene subfamily belong to the b ZIP(the basic domain / leucine zipper)transcription factor family,and they participate in ABA-mediated regulation by regulating the expression of their target genes lead signal transduction pathway.However,there are few reports about ABF / ABI5 / DPBF in cultivated strawberries(Fragaria x ananassa).Whether ABF / ABI5 / DPBF is involved in the development and maturity of strawberry fruits regulated by ABA and how to participate in the development and maturity of strawberry fruits regulated by ABA are still poorly understood less.In this paper,members of the ABF / ABI5 / DPBF subfamily were first identified in cultivated strawberries to explore their spatial and temporal expression characteristics;through analysis of the phenotype and transcription level of transgenic fruits,it was initially clear that FaABI5 is involved in ABA-regulated strawberry fruit development;As a screening system,it was found that the interaction between FaABI5 and Fa Sn RK1(sucrose non-fermenting-1-related protein kinase-1)mediates strawberry fruit development and maturity.The main results are as follows:1.Using the ABF / ABI5 / DPBF amino acid sequences of rice,Arabidopsis and other plants as templates to compare the protein databases encoded by strawberries,five members of the ABF / ABI5 / DPBF subfamily were identified.Phylogenetic tree analysis showed that These subfamily genes are highly related to members of the ABF / ABI5 /DPBF family of Rosaceae fruit trees.2.Analysis of ABF / ABI5 / DPBF subfamily genes during fruit development and organ transcription levels,as well as analysis of transcription levels of different hormone treatments in strawberry fruits,indicates that ABF / ABI5 / DPBF subfamily genes are associated with strawberry fruit ripening correlation.3.Through stable and transient genetic transformation system,stable genetically transformed strawberry seedlings and transiently genetically transformed fruits were obtained;through analysis of phenotypes(fruit weight,fruit color,hardness,soluble solids)and transcription levels of transiently genetically transformed fruits,It is initially clear that FaABI5 is involved in the development and ripening of strawberry fruits regulated by ABA.4.With the help of yeast two-hybrid system,the protein physical interaction between FaABI5 and Fa Sn RK1 was identified through screening.The results suggest that FaABI5 regulates strawberry fruit ripening through Fa Sn RK1.5.The analysis of FaABI5-RNAi transient genetic transformation fruit found that the transcription level of genes related to the phenylpropionic acid metabolism pathway(4CL,CAD,PAL,F3 H,MYB10)was suppressed,and the promoter bases of these genes obtained by chromosome walking technology and the sequence contains ABRE response elements.On this basis,ABF / ABI5 / DPBF protein purification system was established for further research.