The Singnaling Effect Of NO On The Regulation In Maize Root Water Uptake

In this study we used maize as a test material,and using a bioinformatics,physiological and molecular biological methods,we firstly discussed how the NO donors（SNP and GSNO）regulated reactive oxygen species（ROS）in maize roots content and the root hydraulic conductivity rate and the effect of ROS on NO effect of maize root,and the effects of SNP pretreatment on the stress of maize fcacing adversity,in order to provide a theoretical reference of the role NO in the stesses.The main results are as follows:1.We used the knowledge of biological information to find the relationships beteewen animal protein,AQP2 and AQP3,and corn homologous.We found that,in all the corn proteins,homologous relationship of AQP2,AQP3 and PIP proteins is most close to,and they all have a conserved region of NPA.The conserved region is important for the transportation of nutrients and moisture balance.At the same time,because the NO has a role in regulation of AQP2,AQP3,the experimental results show that,NO may also be involved in the NO process of plant water transport.2.Using 20%PEG-6000 as the simulated drought,we explored the effect of NO in drought rewatering on ROS content in root and root water uptake,and the effect of NO pretreatment on drought resistance in plants.The NO donors,SNP and GSNO,can significantly inhibit the maize root hydraulic conductivity,moreover,NO can significantly promote the production of maize root ROS.In addition the H₂O₂ scavenger,can partly inhibit the inhibitory effect of NO on maize root hydraulic conductivity.After SNP pretreatment,NO can reduce the accumulation of ROS in maize root under drought stress,and promoted the root water transport rate.After SNP pretreatment,gene expression of different aquaporins was not the same,and we found that part of the PIPs increased,part of the PIPs delined,and some PIPs did not change.3.After 7 d’s nitrogen deficiency stress,maize was treated with N recovery,and then we join the NO donor and NO scavenger c-PTIO to explore the effect of NO on water absorption and reactive oxygen species in maize roots,and reactive oxygen species in the role of NO in the signaling pathway.The results showed that,in the accession to the NO donors in maize induce a significant decrease in root water uptake after nitrogen appeared,and the content of active oxygen appeared significantly increased.After removal of H₂O₂,CAT had no obvious difference on maize root hydraulic conductivity,but in the NO donor and CAT treatment group,the hydraulic conductance rate significantly increased.Description of NO in the inhibition of corn root can be nitrogen recovery by two ways dependent and does not depend on the H₂O₂ pathway.Using Real-time PCR,we found,after the pretreatment with SNP,six kinds of aquaporins were significantly increased in different degrees.