| Objective: To explore the prevention and treatment effects and mechanism of electroacupuncture on neuropathy in diabetic rats.Provide experimental basis for the clinical application of electroacupuncture and the prevention and treatment of diabetic peripheral neuropathy.Methods: Thirty 8-week-old healthy male SD rats were randomly divided into three groups: 10 diabetes model group(group B),10 electroacupuncture group(group C),and 10 electroacupuncture plus inhibitor group(group D).only.After one week of adaptive feeding,rats in each group were fed with high-fat and high-sugar for another 6 weeks.After the feeding was completed,30mg/kg of streptozotocin(STZ)(concentration 3.0mg/ml)was injected intraperitoneally to create a model.At the same time,group D The rats were intraperitoneally injected with LY294002 solution for 7 consecutive days.Intervention was started after successful detection and modeling: the model rats in group B did not undergo electroacupuncture,but they were bound in the same way as group C and group D each time.Rats in groups C and D were treated with electroacupuncture on both sides of Yushu,Housanli(Zusanli),and Sanyinjiao for 4 weeks,6 times a week.The rats in each group were measured for body weight and tail-tip blood glucose before and after the modeling,and after weekly electroacupuncture intervention.After the intervention,the bilateral sciatic nerves were taken,and one part was fixed with paraformaldehyde for section observation;the other part was taken Then put it into liquid nitrogen and freeze it for detection of protein expression.LFB myelin fast blue staining was used to observe the changes of sciatic nerve myelin in each group;and TUNEL method(notch end labeling method)to detect Schwann cell apoptosis;Western blot(Western Blot,WB)to detect sciatic nerve in each group The expression of PI3 K,AKT,and mTOR in the medium.Results: 1.Body weight: Before STZ injection,there was a significant difference between the model group and the electroacupuncture + inhibitor group(P<0.05),and there was a significant difference between the electroacupuncture group and the electroacupuncture + inhibitor group.(P<0.05);After the modeling is completed,there is a significant difference between the model group and the electroacupuncture + inhibitor group(P<0.05);after the intervention for 4 weeks,there is no significant difference in the weight of the three groups(P>0.05).There was no significant difference in body weight before and after each group of rats(P>0.05);2.Blood glucose: Before and after intervention,there was no significant difference in fasting blood glucose between groups of rats in each group(P>0.05).3.Protein expression: The protein expression of PI3 K,AKT and mTOR in group C was higher than that in groups B and D,and group B was the lowest.4.LFB staining: the sciatic nerve tissue of group B has broken myelin sheath and demyelination;the sciatic nerve tissue of group C has local bending,and the local myelin sheath is rarely broken,and there is no obvious demyelination change;the sciatic nerve tissue of group D has myelin sheathed locally Rupture,accompanied by demyelination.5.TUNEL detection: The apoptosis of rats in group C was less than that in groups B and D,and the apoptosis of rats in group D was slightly less than that in group B.Conclusion: 1.Electroacupuncture at Zusanli,Sanyinjiao,and Yishu has no obvious effect on improving blood sugar in diabetic rats;2.Electroacupuncture can improve Schwann cell apoptosis and sciatic nerve injury;3.Electroacupuncture can increase PI3 K,AKT,The expression of mTOR protein improves nerve damage. |
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