| Objective To investigate the effect of Cepharanthine（CEP）on the proliferation and apoptosis of human pancreatic cancer cell line As PC-1 Bx PC-3 and its mechanism.Methods 2.1875,4.375,8.75,17.5,35μmol·L-1CEP was treated with As PC-1,Bx PC-3 cells were treated with 1.875,3.75,7.5,15,30μmol·L-1CEP for 48 h,and cell proliferation rate was detected by MTT assay.17.5,35μmol·L-1CEP was treated with As PC-1 cells for 48 h,and 15,30μmol·L-1CEP was treated with Bx PC-3 cells for 48 h.Cell morphological changes were observed under an inverted microscope.Cell survival rate was detected by trypan blue staining,cell apoptosis was detected by flow cytometry,and Western blot assay was used to detect changes in expression levels of Cleaved-Caspase3,Cleaved-Caspase9 and signaling pathway associated protein AKT/m TOR.Results Compared with the control group,different concentrations of CEP treated As PC-1 Bx PC-3 cells after 48 h,cell proliferation was inhibited,and the difference was statistically significant（p<0.05）,and the proliferation rate of As PC-1,Bx PC-3cells decreased gradually with the increase of the dose of CEP.The cell morphology of As PC-1,Bx PC-3 cells was significantly changed after treated with low and high concentration CEP for 48 h.Trypan blue staining showed a significant decrease in cell viability with increasing dose（p<0.05）;The results of flow cytometry showed that the apoptosis rate was increased in all the treatment groups compared with the control group,and the higher the dose was,the more significant the apoptosis rate was（p<0.05）;Western blot results showed that the Cleaved-Caspase3 Cleaved-Caspase9apoptotic proteins were upregulated after CEP treatment.Signaling pathway related proteins p-AKT,p-m TOR were down-regulated（p<0.05）.Conclusion CEP can inhibit the proliferation of human pancreatic cancer As PC-1,Bx PC-3 cells and induce their apoptosis.The mechanism of inhibition of proliferation and apoptosis may be related to the inhibition of AKT/m TOR signaling pathway. |
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