| Every year,there will be a large number of patients suffering from skin defects caused by burns,surgical treatment,traffic accidents,etc.However,the skin repair technologies are still inadequate.Skin is a complex structure that is consisted of epidermis,dermis,hair follicles,sweat glands and some other layers.The best skin repair technology should reconstruct these structures and functions.The in-situ 3D bioprinting technology can conduct 3D model based on the skin defect site of the patient,simulate the real tissue structure of the skin,and directly carry out 3D printing repair on the skin defect site,providing the possibility of rapid,personalized and highly simulated repair of skin defect.This paper focuses on the key problems that need to be solved by in-situ 3D bioprinting technology,which can quickly and intelligently identify the wound,locate the printing area according to the wound and the layers of skin structure,repair with a variety of cells and materials.Around these objects,we have done the following explorations.1.Make a deep learning model to recognize the diseased skin parts.2.Develop multi-channel biological 3D printing system to realize the collaborative printing of various cells,materials.3.Isolate and culture the seed cells of skin tissue,config cell&biomaterial inks.4.Print multi-layer artificial skin tissue.5.Evaluate the structure and functions of the printed skin tissue.The results showed that AI technology can easily recognize and segment the injured skin,which achieved 84%accuracy of segmentation.The multichannel 3D bioprinting system can efficiently and accurately print artificial skin structure build with multi-cells&biomaterials.The isolated epidermic cells and fibroblast can grow rapidly.The structure of 3D-printed skin is similar to human skin tissue.The test results of skin barrier function,irritation,light damage and other indicators proved that the function of printed skin is close to human skin. |
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