Preliminary Study On The Mechanism Of Monensin On Breast Cancer Cells MDA-MB-231

Cancer is a major cause of death in our country.Breast cancer is a malignant tumor with the highest incidence of women worldwide,and the incidence of male breast cancer is also increasing year by year.Breast cancer has now replaced lung cancer,becoming the cancer with the highest incidence in the world.Monensin is a polyether ion compound extracted from Streptomyces cinnamon,which can inhibit the growth and reproduction of bacteria by changing the concentration of H⁺,Na⁺and K⁺in bacterial cells.Studies have shown that monensin can inhibit the growth and proliferation of a variety of tumor cells,and induce the apoptosis of a variety of tumor cells.So far,the effect of monensin on human breast cancer cell MDA-MB-231 has not been reported.Therefore,this study explored the proliferation,apoptosis and preliminary mechanism of monensin on human breast cancer cell MDA-MB-231.Different concentrations of monensin were used to treat human breast cancer cells MDA-MB-231 cultured in vitro,and MTT method,cell counting method,colony formation experiment and scratch experiment were used to detect the effect of monensin on the proliferation and migration of breast cancer cells MDA-MB-231.Giemsa and acridine orange staining,lactate dehydrogenase kit,agarose gel electrophoresis experiments were used to detect the effect of monensin on the apoptosis of breast cancer cells MDA-MB-231.Flow cytometry was used to detect the effect of monensin on the apoptosis and cell cycle of MDA-MB-231 cells.Reactive oxygen kit was used to detect the effect of monensin on the level of active oxygen in MDA-MB-231 cells.Caspase kit was used to detect the effect of monensin on the activity of caspase in MDA-MB-231 cells.Western blot experiment was used to detect the effect of monensin on the expression of apoptosis-related proteins and autophagy-related proteins in MDA-MB-231 cells.The results showed that 2‰of solvent ethanol had no significant effect on cell survival rate.Monensin can inhibit the proliferation of breast cancer cells MDA-MB-231 in a time-and dose-dependent manner.Monensin can block the cell cycle of MDA-MB-231 in G₀/G₁ phase.Monensin can increase the level of reactive oxygen species in MDA-MB-231 cells and induce cell apoptosis,which is related to the significant increase in the activity of caspase-3/4/8/9 enzymes.Monensin can increase the expression of apoptosis-related proteins Bax,Cyt-C and autophagy-related proteins Atg5,Atg7,Beclin-1 and ULK1.In summary,monensin could inhibit the proliferation of breast cancer cellsMDA-MB-231,induce apoptosis and autophagy of breast cancer cells MDA-MB-231,and block the cell cycle in G0/G1 phase.