Analysis Of Gut Microbiome Structure Differences In Different Body Lengths Of Zebrafish Based On 16S RDNA Gene Sequencing Technology

Background: Gut Microbiome is a complex microbial community colonized in the gastrointestinal tract,which is closely related to the occurrence and development of many human systemic diseases.Recent studies have found that gut microbiome can regulate the relative activity of osteoclasts and osteoblasts through a variety of ways and affect bone metabolism and growth.Including the production of self metabolites,the change of host nutritional metabolism level,and the regulation of mucosa and immune response system,but its specific mechanism is still unclear.Zebrafish is highly homologous with human genes.At the same time,zebrafish also has high similarity with human genes and bones;Its embryo is transparent and develops rapidly;Compared with traditional mice,rabbits and mammals,it has incomparable advantages such as fast reproduction,short cycle,low cost,high biosafety and high-throughput drug screening.It has been widely used in the research of related diseases and therapeutic drugs such as osteoporosis,osteoarthritis,bone tumor.Therefore,a better understanding of the structural and functional changes of intestinal flora will help us to find new biomarkers and understand the interaction between intestinal flora and bone mass.Objective: Based on 16 S r DNA sequencing technology,this study analyzed and compared the changes of the structure and abundance of gut microbiome of zebrafish with different body lengths,so as to provide a new idea for exploring the relationship and mechanism between gut microbiome and bone development.Methods: Zebrafish of the same parental generation aged 3 months and 6 months were divided into long body group and short body group according to body length.The composition of gut microbiome of zebrafish was detected by 16 S r DNA sequence analysis,the diversity and abundance of gut microbiome of zebrafish with different body length were analyzed and compared between the two groups.Results: 1.Alpha diversity analysis showed that the abundance and diversity of flora in the long body group were significantly higher than those in the short group of 3-month-old zebrafish.2.Beta diversity analysis could discriminate the gut microbiome of long body group from the short group.Among the dominant bacteria of 6-month-old zebrafish,there was no significant difference between the male long group and the short group;There were significant differences in Proteobacteria and Fusobacteriota between the female long group and the short group.3.Linear discriminant analysis showed that seven phyla communities and three phyla communities were enriched in the long group and short group at different months of age,respectively.Thirteen bacterial communities and seven bacterial communities were enriched in the long group and short group at different months of age,respectively.Conclusion: The body length of zebrafish is closely related to the diversity of gut microbiome.Regulating the structure and abundance of gut microbiome is a potentially effective strategy to promote skeletal development.