| Hernia is a high incidence in general surgery,and adults can’t heal themselves.The only effective treatment is to implant hernia repair mesh to strengthen the tissue of defect or weak part.As a typical implantable biomedical textile,mesh mainly includes synthetic mesh and biological mesh.In view of the limitations of the source and price of biological mesh,synthetic mesh always occupies the main market.Although synthetic mesh can temporarily replace abdominal wall tissue and provide sufficient mechanical strength,there is serious inflammatory foreign body reaction between synthetic material and body.Patients suffer from postoperative complications such as chronic pain,shrinkage and adhesion.Therefore,exploring effective anti-inflammatory strategies of hernia repair mesh not only has important social value,but also has far-reaching significance for promoting the clinical application of biomedical textiles.Based on the background,starting from the first step of interaction between material and non-specific protein adsorption,this study constructed an anti-fouling surface that could reduce protein adsorption.The surface of polypropylene（PP）mesh was activated by dopamine,and initiated by free radical polymerization of tertiary amine / photoinitiator,Poly（carboxybetaine methacrylate）（PCBMA）coating was directly polymerized and grafted on the surface of the mesh.Firstly,the feasibility of the reaction system was verified by Fourier Transform InfraRed（FTIR）and X-ray photoelectric spectroscopy;Then the effects of monomer concentration and reaction time on the grafting effect were investigated.The best experimental parameters of grafting reaction were selected through the test results of FTIR,water contact angle（WCA）and scanning electron microscope;The prepared PCBMA-PP mesh was tested for monofilament diameter,gram weight,porosity,axial tension,suture pull and tear to characterize the effect of modification process on the physical and mechanical properties of the mesh;The antifouling properties of zwitterionic grafted mesh were investigated by the characterization of WCA,surface charge,thickness,hydration lubricity and anti-protein adsorption.In addition,the stability of antifouling coating was also tested;the biocompatibility and inflammatory response of the mesh were characterized by cell experiment,finally,the control effect of mesh on inflammation in vivo was tested by animal experiment.The results show that the surface grafting technology used in this paper successfully can obtain PCBMA-PP modified mesh.The grafting process of zwitterionic monomer on the surface is affected by monomer concentration and reaction time.With the increase of monomer concentration and reaction time,the deposition of polymer on the surface of monofilament increases;Although the coating formed by PCBMA completely covers the monofilament,the test results of physical structure characterization,axial tensile properties,suture tensile properties and tear properties show that the addition of the coating does not change the basic structure of the mesh,and the mechanical properties of the mesh are retained;According to the antifouling performance of the PCBMA-PP mesh,the addition of PCBMA improves the hydrophilicity of PP mesh and reduces the surface charge of mesh.The cationic and anionic groups of PCBMA attract the hydrated layer formed by water molecules to bring surface lubricity to the mesh,thus reducing the protein adsorption on the surface of mesh,PCBMA coating shows good stability in static degradation test and dynamic tensile test in vitro;in the cell experiment,PCBMA-PP mesh has good cytocompatibility and can reduce the expression of macrophage proinflammatory factor.The final animal experiment shows that PCBMA-PP mesh has a good anti-inflammatory effect in vivo. |
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