Expression Of Acid-sensing Ion Channels In Gastric Mucosa Of Patients With Helicobacter Pylori And Its Role In Gastrointestinal Function

Background and Objective: Chronic gastritis is one of the most common serious pandemic infections in the world,which poses a great threat to human health.Most children’s gastritis is caused by Helicobacter pylori（HP）infection,if not treated in time,may lead to a series of complications such as peptic ulcer or gastric cancer in old age.It has been confirmed that acid-sensing ion channels（ASICs）are involved in the development of functional gastrointestinal diseases.However,the pathogenesis of Helicobacter pylori-associated gastritis in children has not been elucidated.The purpose of this study was to investigate the expression of acid-sensing ion channels in gastric mucosa of patients infected with Helicobacter pylori and the role of its expression in the gastrointestinal function of gastritis with H.pylori infection.Methods: 22 patients with gastritis diagnosed via endoscopy were divided into 2 groups:the Helicobacter pylori positive group（Hp（+）,n=11）and the Helicobacter pylori negative group（Hp（-）,n=11）.The expression and localization of ASICs in gastric mucosa from patients with gastritis were examined using immunochemical techniques.Serum CCK,SP,GAS and MTL levels were measured with ELISA methods.The correlation between ASICs expression and gastrointestinal hormones was investigated by Pearson’s analysis.Results: ASIC3 expression was significantly increased in the gastric mucosa of patients with Hp infection,and serum CCK,SP and MTL levels were obviously higher in patients with Hp.Moreover the serum CCK,SP and MTL levels were closely correlated to ASIC3 expression.Conclusion: Hp could induce the expression of ASIC3 in gastric mucosa,which affects the secretion of gastrointestinal hormones and gastrointestinal function in patients infected with Hp.