| Background Uterine corpus endometrial carcinoma（UCEC）is a common malignant tumor of female reproductive system.In recent years,the incidence of uterine corpus endometrial carcinoma is on the rise,and the onset age tends to be younger.UCEC originates from endometrial glands,of which uterine endometrioid adenocarcinoma（UTEA）is the most common pathological type.According to the histological structure and nuclear characteristics,UCEC is divided into 3 grades in 2009 FIGO standard: High differentiation（grade I）,medium differentiation（grade II）,and low differentiation（grade III）.At present,surgical resection of the whole uterus,bilateral fallopian tubes and ovaries is the preferred treatment for early UCEC,and systemic chemotherapy is the preferred treatment for late/recurrent UCEC.For some female patients with fertility requirements,surgical contraindications or lost surgical opportunities,searching for ideal molecular markers is of great significance for non-surgical treatment and prognosis assessment of UCEC.Pleomorphic adenoma gene like-1（PLAGL1）is a member of pleomorphic adenoma gene（PLAG）family,encoding zinc finger protein of the same name.Blocking cell cycle and mediating apoptosis through various pathways are closely related to various physiological functions and pathological states of cells.Estrogen receptor 2（ESR2）is a member of estrogen receptor family and nuclear receptor transcription factor superfamily,encoding estrogen receptor β（ERβ）,binding to 17-estradiol or related ligands inhibits the activity of other estrogen receptor family members,and in UCEC exerts tumor inhibition by antagonizing estrogen.In this study,we investigated the expression of PLAGL1 and ERβ in UTEA and their correlation with the clinicopathological characteristics of patients,in order to provide reference for the early diagnosis,personalized treatment and prognosis evaluation of UTEA.Purpose1)To investigate the expression of PLAGL1 and ERβ in UTEA tissues and their relationship with clinicopathological characteristics of patients.2)To analyze the correlation of PLAGL1 and ERβ expression levels in UTEA.Method1)Differentially expressed genes were screened in UCEC and normal endometrial tissues from the Cancer Genome Atlas（TCGA）,and analyzed the expression of PLAGL1 and ESR2 m RNAs in UCEC and normal endometrial tissues using UALCAN database.2)Paraffin samples were collected from 84 cases of UTEA and 43 cases of normal endometrium adjacent to cancer.The expression of PLAGL1 and ERβ in paraffin samples was detected by immunohistochemistry,and the correlation between PLAGL1 and ERβ expression levels in UTEA was analyzed.Result1)In UCEC,the expression of PLAGL1 m RNA was significantly lower than that in normal endometrium（P<0.01）,but there was no significant difference in ESR2 m RNA expression between normal endometrium and UCEC（P>0.05）.2)In UTEA,the PLAGL1 protein high expression rate was 34.5%,which was significantly lower than that in adjacent tissues（74.4%,P<0.01）.The high expression rate of ERβ was 39.3%,and there was no significant difference between ERβ and para-cancer tissues（34.9%,P>0.05）.3)The expression of PLAGL1 protein was significantly correlated with UTEA tumor grade,clinical stage,cervical involvement,muscle layer invasion depth,lymph node metastasis and p53 missense mutation（all P<0.05）,which was not associated with age and menopausal status（all P>0.05）.The expression level of ERβ was not correlated with UTEA patients’ age,menopausal status,tumor grade,clinical stage,degree of cervical involvement,depth of muscular invasion,lymph node metastasis and p53 missense mutation（all P>0.05）.4)In UTEA,PLAGL1 was positively correlated with ERβ expression（r=0.236,P<0.05）.Conclusion The expression level of PLAGL1 protein in UTEA was significantly decreased,which can be used as a molecular marker for pathological diagnosis and prognosis assessment of UTEA.The abnormal expression of PLAGL1 and ERβ may be involved in the development and progression of UTEA. |
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