Study On The Characteristic Of Gut Microbiota In Patients With Neuromyelitis Optica Spectrum Disorders Based On 16SrDNA Sequencing

Objects:To investigate the characteristics of gut microbiota in patients with neuromyelitis optica spectrum disorder（NMOSD）,to evaluate the differences in gut microbiota composition between NMOSD patients and healthy individuals,and to in-depth study of the relationship between gut microbiota and NMOSD.For the purpose of providing some evidence-based medical evidence for gut microbiota as a potential non-invasive biomarker for the diagnosis of NMOSD.Methods:The stool samples were collected from 11 NMOSD patients in acute exacerbation and 20 healthy volunteers.16 S ribosomal DNA high-throughput sequencing targeting V3-V4 hypervariable region sequencing technology was used to detect the composition and structure of gut micribiota in the two groups.Finally,the bioinformatics analysis included the diversity of microbiota,species differences and function prediction were performed.Results:There was no significant difference in the overall microbial community between the NMOSD group and control group,but the gut microbiota compositions was significantly different.Compared with the control group,the abundance of Proteobacteria in the NMOSD group was significantly increased and the relative abundance of faecalibacterium,Collinsella and Alistipes was decreased in the NMOSD patients.The NMOSD group had an increased abundance of Enterobacter,Escherichia-Shigella and Weissella compared to healthy control group.Functional prediction analysis showed that the gut microbiota of NMOSD patients were related to three signaling pathways: “carbohydrate metabolism”,“cofactor and vitamin metabolism”and “lipopolysaccharide metabolism and biosynthesis”.Conclusions:1.The composition of microbiota was significantly different between NMOSD group and the control group（P<0.01）.Compared with the control group,the abundance of faecalibacterium,Collinsella and Alistipes were decreased in NMOSD group;the number of Enterobacter,Escherichia-Shigella,Weissella were increased significantly.2.There are differences between NMOSD group and control group in the signaling pathway related to functional prediction analysis.The signaling pathway of NMOSD group may be related to the metabolic pathways of “gut brain axis”.3.The dysbiosis of gut microbiota may be related to the pathogenesis of NMOSD.4.The gut microbiota may serve as a potential biomarker for the diagnosis of NMOSD and a target for individualized treatment in the future.