| PurposeFibrinogen-like protein 1（Fibrinogen-like protein 1）in normal cervical tissues,high-grade Squamous intraepithelial lesion（HSIL）tissues and cervical cancer tissues were detected.Combined with clinicopathological data,the expression level of FGL1 in cervical cancer was analyzed to explore the expression of FGL1 in the occurrence and development of cervical cancer and its correlation with clinical characteristics and prognosis,in order to lay a foundation for further improving the survival rate of patients with cervical cancer.MethodsPatients who were collected in this hospital from January 2010 to January 2015（40 patients）.The normal cervical tissue was used from patients undergoing total hysterectomy for benign uterine lesions（10）;HSIL from patients（10）;Patients with cervical squamous cell carcinoma（15 patients）and adenocarcinoma tissue（5 patients）undergoing radical cervical cancer are stage I and Ⅱaccording to FIGO stage（International Federation of Gynecology and Obstetrics,FIGO）.The 5-year survival rate of patients with the end of the experimental day or the follow-up as the follow-up end point.Paraffin sections were prepared for paraffin sections and FGL1 expression was detected by immunohistochemistry and group differences were analyzed statis.The correlation between THE expression of FGL1 and the clinicopathological parameters of cervical cancer（age,FIGO stage,degree of differentiation,lymph node metastasis）was analyzed by statistical method..The Kaplan-Meier method was used to evaluate the relationship between FGL1 protein expression levels and the prognosis of cervical cancer patients and to plot the survival curves.Result1.Immunohistochemical staining analysis: no obvious positive expression of FGL1 in normal cervical tissue;FGL1 was high in cervical HSIL tissue,positive rate of60.00%;FGL1 high in cervical squamous SCC tissue,positive rate of 86.67%,and FGL1 positive in cervical adenocarcinoma tissue of 80.00%.2.For data analysis using Fisher’s exact probability test,FGL1 expression in four different cervical tissues with P <0.05 was statistically significant.3.In cervical squamous cell carcinoma tissue,the high expression rate varied by age and FIGO stage.Data analysis with Fisher’s exact probability test set P <0.05.The results showed that FGL1 was correlated with the histological parameters of cervical squamous cell carcinoma,but not with the patient’s age,clinical stage,lymph node metastasis and other pathological data,and the difference was not statistically significant4.A total of 15 patients with cervical squamous cell carcinoma were followed up by telephone or outpatient for 5-year survival,including 6 patients who were lost to follow-up and 9 patients who had an outcome,including 3 patients with low and medium expression of FGL1 and 6 patients with high expression.The Kaplan-Meier method was used to describe the correlation between FGL1 protein expression and5-year survival in SCC and analyze survival characteristics,comparing the survival curves between FGL1 and high expression in SCC by Log rank test,high FGL1 expression was significantly associated with survival within 5 years,X2 =5.376,P=0.02,<0.05.Conclusion1.FGL1 is highly expressed in cervical cancer tissue and varies from normal cervical tissue and high-grade cervical squamous intraepithelial lesion tissue（HSIL）.2.FGL1 expression may be negatively correlated with the degree of cervical squamous cell carcinoma tissue differentiation,but not with other pathological parameters: age,FIGO stage,and lymph node metastasis.3.High FGL1 expression may be associated with increased survival in patients with cervical SCC within 5 years. |
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