Study On The Processing Technology And Quality Material Basis Of Honey Processed Polygonati Rhizoma

ObjectiveThe honey processed Polygonati Rhizoma was first published in the Qing Dynasty Mujing Dacheng.The honey processed Polygonati Rhizoma has an enhanced lung nourishing power,it is good at treating dry lungs and dry coughs.Honey processed Polygonati Rhizoma as a traditional Chinese medicine,the preparation method described in herbal medicine can be further optimized and improved.In this experiment,through the use of modern scientific means,the processing technology,quality control methods,changes of main chemical components before and after processing were preliminary studied,and the processing and acting mechanisms of honey processed Polygonati Rhizoma were also predicted.Methods1.Single factors are used to conduct preliminary investigations on the degree of refining honey,the ratio of material to liquid,the moistening time,the stewing time,and the stewing firepower of the honey processed Polygonati Rhizoma(Polygonatum cyrtonema Hua).It is combined with the orthogonal test method,and the content of the internal components(polysaccharides,total Saponins and diosgenin)and the pharmacodynamics(cough relieving)are used as evaluation indicators to further investigate the material-to-liquid ratio,moistening time,and stewing time to establish the best processing technology.2.This experiment investigate the sterilization methods of honey processed Polygonati Rhizoma through dry heat sterilization,moist heat sterilization and ultraviolet sterilization.The best sterilization method of honey processed Polygonati Rhizoma was selected based on the content of internal components(polysaccharides,total saponins and diosgenin)and microbial content.3.By studying the characteristics,microscopic and TLC identification,water content,ash content,microbial limit,extract and the content of Polysaccharides and Diosgenin of different batches of honey processed Polygonati Rhizoma,the quality standard of honey processed Polygonati Rhizoma was initially established.4.In this experiment,HPGPC,PMP-HPLC derivatization,Seliwanoff method,FT-IR,NMR and other technologies were used to preliminarily identify the structure of Polygonatum polysaccharide before and after honey processing;HE staining,Elisa,PCR and other techniques were used to study the protective effect of polysaccharides before and after honey processing Polygonati Rhizoma on acute lung injury mice,and to analyze the action principle and processing mechanism of the processing of honey processed Polygonati Rhizoma.Results1.Polygonati Rhizoma pieces plus 20% refined honey,airtight and moisturized for 16 hours until the liquid auxiliary materials are basically absorbed,put in a stewing container,and heat and stew with circulating steam at 100°C for 16 hours.2.From dry heat sterilization,moist heat sterilization and ultraviolet sterilization,moist heat sterilization is selected as the best sterilization method for honey processed Polygonati Rhizoma.3.The properties,microscopic identification and thin-layer identification of the prepared honey processed Polygonati Rhizoma should meet the specified requirements.It is recommended that the moisture content in the honey processed Polygonati Rhizoma standard should not exceed 15.0%;the total ash content should not exceed 4.0%;the microbial content should comply with the "Microorganism Limit Standard for Non-sterile Drugs(General Principle 1107)" in the 2020 Chinese Pharmacopoeia;the content of extract should not be less than 63.0%;the content of honey processed Polygonati Rhizoma polysaccharides should not be less than 4.0%,and the content of diosgenin should not be less than 0.12%.4.HPGPC was used and found that the main molecular weight of Polygonati Rhizoma polysaccharide(PCP)was 8.842×103Da,and the main molecular weight of honey processed Polygonati Rhizoma polysaccharide(HPCP)was 5.521 × 103Da;PMP-HPLC derivatization and Seliwanoff method were used to speculate that PCP is mainly composed of fructose(92.73%),Glucose(6.37%),galactose(0.90%),HPCP is mainly composed of fructose(60.16%),glucose(22.35%),galactose(13.03%),arabinose(3.12%),xylose(1.35%);FT-IR is used to speculate that polysaccharides before and after processing contain pyranose and fructose;NMR is used to speculate that PCP may contain 3 sugar residues and HPCP may contain 5 sugar residues,PCP and HPCP both contain α-and β-pyranose,with α-D-Glcp residues,furanose,fructose,galactose,no uronic acid and rhamnose.HE staining,Elisa,PCR and other techniques were used to detect the degree of lung edema,lung tissue pathology,inflammatory factors(IL-1β,IL-6 and TNF-α)content in BALF,and the content of oxidative stress-related enzymes(MPO,SOD)in lung tissues,the m RNA expression of inflammatory factors(IL-1β,IL-6 and TNF-α)and antioxidant enzymes(Nrf2 and its downstream genes HO-1 and NQO-1)in lung tissues showed that PCP and HPCP had protective effects on mice with acute lung injury,and HPCP had better effects.Therefore,one of the principles of the processing of honey processed Polygonati Rhizoma may be that the structure of Polygonati Rhizoma Polysaccharides changes after honey processing,and the protective effect on the lungs is enhanced.Conclusion:This experiment conducted a systematic study on the processing technology,sterilization methods,quality control of honey processed Polygonati Rhizoma,and the structure and protective effect on the lungs of Polygonati Rhizoma polysaccharides before and after processing.Provide reference and basis for the follow-up experiment,modern production and clinical drug of honey processed Polygonati Rhizoma.