Relationship Between Dysbiosis Of Oral And Gut Microbiota In Patients With Neuroendocrine Tumors Of Colorectum Or Pancreas

Aim: The aim of this study is to investigate the dysbiosis of the oral and gut microbiota in the patients with colorectal,or pancreatic neuroendocrine tumor,looking for potential biomarkers of the NEN clinical diagnosis and prognosis evaluation provides new means.Methods: Pancreas NENs group were selected from the gastroenterology and nuclear medicine department of Nanjing Hospital Affiliated to Nanjing Medical University from November 2019 to July 2020 and classified into colorectum NENs group(C)and pancreas NENs group(P)according to different tumor sites.Results:1.Species diversity: The species abundance and diversity of the bacterial flora on the tongue,saliva and feces of patients with colorectal and pancreatic NENS were higher than those of healthy controls.The species abundance and diversity of fecal flora in patients with colorectal and pancreatic NENS were the highest,followed by saliva and tongue coating.2.Species composition:(1)Tongue: The dominant bacteria of the tongue coating flora of colorectal and pancreatic NENs patients and healthy people included Haemophilus,Neisseria,Streptococcus,Prevotella＿7,Fusobacterium,Prevotella,Veillonella,Leptotrichia,Prevotella,Actinomycetes,Rothia,Porphyromonas,etc.The abundance of streptococcus in the tongue of colorectal and pancreatic NENs patients was higher than healthy people,and the abundance of streptococcus in the colorectal NENs group was higher than that of pancreatic NENs group.The abundance of Prevotella,Alloprevotella and Fusobacteria in patients with colorectal and pancreatic NENs was lower than healthy people.(2)Saliva: The dominant bacterial in the salivary flora of colorectal and pancreatic NENs patients and healthy people include Neisseria,Streptococcus,Haemophilus,Prevotella＿7,Rothia,Veillonella,Fusobacteria,Leptotrichia,Prevotella,Actinomycetes,Porphyromonas,etc.The abundance of Leptotrichia,Actinobacillus,Gemella and Lactobacillus in the salivary flora of patients with colorectal and pancreatic NENs was higher than that of healthy people,and the abundance of Lactobacillus in the salivary flora of patients with colorectal NENs was higher than patients with pancreatic NENs.The abundance of Haemophilus,Alloprevotella and Fusobacteria in the salivary flora of patients with colorectal and pancreatic NENs was lower than that of healthy people,and the abundance of Fusobacteria in the salivary flora of patients with pancreatic NENs was lower than patients with colorectal NENs.(3)Feces: The dominant bacterial in the fecal flora of colorectal and pancreatic NENs patients and healthy people include Bacteroides,Faecalibacterium,Escherichia-Shigella,Roseburia,Megasphaera,Megamonas,Veillonella,Dialister,Eubacterium＿coprostanoligenes＿group,Blaautia,Klebsiella,etc.The abundance of Megamonas and Ruminococcus＿torques＿group in the fecal flora of colorectal and pancreatic NENs patients was lower than healthy people.3.Dominant bacteria of each group(1)CT: Leptotrichia,Actinomycetes,Atopobium,lactobacillus,Prevotella＿2,Aggregatibacter.(2)PT: Yersinia,Acetitomaculum.(3)HT: Fusobacteria,Prevotella,Actinobacteria.(4)CS: Streptococcus,Lactobacillus,Gemella.(5)PS: Rothia,Leptotrichia,Actinomycetes.(6)HS: Haemophilus,Prevotella,Alloprevotella,Prevotella＿7,Fusobacteria,Lautropia.(7)CF: Eubacterium＿coprostanoligenes＿group,Anaerotruncus,Ruminococcaceae＿UCG＿002,Christensenellaceae＿NK4A214＿group,Ruminococcaceae＿UCG＿005,Ruminiclostridium＿9,Ruminococcaceae＿UCG＿010.(8)PF: Megasphaera,Fusobacterium,Prevotella＿7,Neisseria.(9)HF: Megamonas,Klebsiella,Haemophilus,Ruminococcus＿torques＿group,Erysipelatoclostridium,Erysipelotrichaceae＿UCG＿003,Eubacterium＿Ruminantium＿group,Coprococcus＿3,Clostridium＿Sensu＿Stricto＿1,Peptoclostridium.4.Dominant bacteria of NENs T,NENs S,and NENs F(1)NENs T: Prevotella＿7,Haemophilus,Leptotrichia,Fusobacteria,Veillonella,Actinomyces,Prevotella,Porphyromonas.(2)NENs S: Streptococcus,Neisseria,Rothia,Alloprevotella,Actinomycetes.(3)NENs F: Bacteroides,Escherichia-Shigella,Preprevella＿9,Eubacterium＿coprostanoligenes＿group,Lachnoclostridium,Megasphaera,.Conclusions:1.The tongue coating,saliva and fecal microbiota of patients with colorectal and pancreatic NENS were disregulated,and the species abundance and diversity were higher than those of healthy people.2.There were significant differences in the community structure of tongue coating,saliva and fecal microbiota in colorectal and pancreatic NENS patients.Species abundance and diversity of fecal microbiota were the highest,followed by saliva and tongue coating.3.The pathogenesis of NEN is unknown and its relationship with the flora is not clear.The increase in the abundance of Actinobacteria and the decrease in the abundance of Haemophilus may have potential significance for the disease status of NEN.