Study On The Therapeutic Effect And Mechanism Of Yurui Enema Decoction On Rats With Ulcerative Colitis

Objective:To observe the effect of Yurui Enema Decoction on colonic mucosal tissues and related cellular inflammatory factors in rats with ulcerative colitis,so as to preliminarily explore the mechanism of Yurui Enema Decoction on PI3K/ Akt signaling pathway.Methods: In this study,the TNBS(2,4,6-trinitrobenzenesulfonic acid)/ ethanol method was used to induce the establishment of a rat model of ulcerative colitis,and the rats were treated with high,medium and low doses of Yurui Enema Decoction,Continuous administration for 14 days.(1)Observe the daily changes of rats in each group;(2)HE staining to observe the pathological changes of colon;(3)ELISA method to detect the contents of TNF-α,IL-6,IL-10 in rat colon tissue;(4)RT-PCR to detect PI3 K in colon tissue,AKT and other gene expression levels;(5)Western Blot method to detect PI3 K,AKT protein expression levels in colon tissue.Results:(1)Observation of general conditions: rats in the model group were sluggish,activity and eating were significantly reduced,weight loss,pus,blood,mucus,etc.were visible to the naked eye;compared with the model group,rats in each administration group had different degrees of blood and mucus in the early stage Stool,accompanied by weight loss,thinning of hair,etc.After the intervention,the related symptoms of each administration group were significantly improved,among which the weight gain and the improvement of stool characteristics were particularly prominent.(2)Colonic histopathological changes and scores: In the model group,there was a large area of ulcers in the intestinal mucosal layer,the injury could reach the muscle layer,the mucosal structure was basically lost,accompanied by inflammatory cell infiltration,and the DAI score increased(P<0.05);and the model group In comparison,the mucosal injury of colon tissue in each administration group was improved to different degrees,mucosal congestion and edema were reduced,and inflammatory cell infiltration was reduced;among them,the DAI scores of the positive drug group and the high and medium dose groups of Yurui Enema Decoction showed different degrees The down-regulation(P<0.05).(3)The contents of TNF-α,IL-6,and IL-10 in the colon group: The contents of pro-inflammatory factors TNF-α and IL-6 in the model group increased,while the contents of anti-inflammatory factor IL-10 decreased significantly(P<0.05);The administration group had different degrees of down-regulation of TNF-α content and different levels of up-regulation of IL-10 content(P<0.05).Among them,the high and medium dose group of Yurui Enema Decoction and the positive drug group had down-regulation of IL-6 content.Obviously(P<0.05).(4)PI3K and AKT gene expression levels in colon tissue: The expression levels of PI3 K and AKT genes in the model group were significantly increased(P<0.05);the expression levels of AKT genes in each administration group were downregulated(P<0.05),among which Yurui Enema Decoction high and medium dose groups and positive drugs significantly down-regulated the expression of PI3 K gene(P<0.05).(5)The expression levels of PI3 K and AKT proteins in colon tissue: The expression levels of PI3 K and AKT proteins in the model group were significantly up-regulated(P<0.05);the expression levels of PI3 K and AKT proteins in each administration group were down-regulated to varying degrees(P<0.05).Conclusions: Yurui Enema Decoction has a certain intervention effect on ulcerative colitis: it can inhibit the release of pro-inflammatory cytokines TNF-α and IL-6,and at the same time stimulate the production of anti-inflammatory cytokine IL-10,and regulate the relationship between inflammatory cytokines.The balance thus relieves intestinal inflammation damage and exerts an anti-inflammatory effect.The intervention mechanism of Yurui Enema Decoction on ulcerative colitis: its mechanism of action may be related to the PI3K/AKT signaling pathway.It can effectively inhibit the activation of PI3 K and AKT,thereby reducing the production of downstream related cytokines and inflammatory mediators,and alleviating the intestinal tract Inflammation to achieve the purpose of intervention.