Changes Of Intestinal Flora In Patients With Chronic Atrophic Gastritis And Observation On Intervention Effect Of Jian Pi Hua Yu Jie Du Recipe

ObjectiveThe 16SrDNA high-throughput sequencing technology was used to compare the differences in intestinal flora between patients with chronic atrophic gastritis(CAG)and healthy people,and observe the clinical efficacy and the change of intestinal flora on CAG patients by Jian pi hua yu jie du recipe,and explore the potential relationship between CAG and intestinal flora.Method61 patients with CAG(spleen deficiency and stasis syndrome)were included as the experimental group(CAG),and 62 healthy volunteers as the control group(NOR).To explore the composition and differences of intestinal flora between CAG and NOR,collecting and sequencing fecal samples by 16 Sr DNA high-throughput sequencing technology.22 CAG patients were treated with the prescription of Jian pi hua yu jiedu recipe.After the 12-week course of treatment,we need to evaluate the clinical efficacy and the change of intestinal flora.Result(1)Comparison of intestinal flora between CAG and NOR : the Chao1 and ACE of CAG were lower than NOR,and Simpson was higher than that of NOR,indicating that the flora richness and diversity of CAG patients were lower than healthy people.PCA and PCo A analysis showed that the composition of microbial community in CAG was similar to that in NOR,while the diversity of species structure was significantly different between groups.Bacteroidetes and Proteobacteria at the level of phylum,Bacteroidia,Gammaproteobacteria,Bacilli at the level of class,Bacteroidales,Enterobacteriales,Lactobacillales at the level of order,and Bacteroidales at the level of class,respectively.Ruminococcaceae,Prevotellaceae,Enterobacteriaceae,Streptococcaceae at the family level.In the genus level,Bacteroides,Faecalibacterium,Escherichia-Shigella,Roseburia and Prevotella-2 had statistical significance(P < 0.05).(2)The total effective rate was 91.09% by using the Jian pi hua yu jie du recipe,After treatment,the total score of TCM syndrome significantly lower(P< 0.01),the syndrome of fullness,stomachache,anorexia,fatigue relieved after treatment(P<0.01),the syndrome of loose stools,bitter taste relieved after treatment(P< 0.05),gloomy complexion had no rectify(P> 0.05);There were significant differences in pathological scores before and after treatment(P<0.01),the grade of atrophy,intestinal metaplasia,the scores of inflammation and inflammation activity were lower after treatment(P< 0.05).(3)Comparison of intestinal flora before and after treatment with the Jian pi hua yu jie du prescription: Chao 1 index after treatment was less than that before treatment,and residual α diversity index was insignificant;PLS-DA and NMDS analysis showed that there was a difference in bacterial diversity before and after treatment,but the difference was not significant.At the phylum level,the abundance of Bacteroidetes was up-regulated,while the abundance of Proteobacteria was down-regulated.At the familylevel,the proportion of Lachnospiraceae,Bacteroidaceae and Bifidobacteriaceae was increased,while the proportion of Fusobacteriaceae and Streptococcaceae was decreased.Had statistical significance(P < 0.05);After LEf Se,Bacteria enriched in the intestines of CAG patients before treatment included Bacillales,Staphylococcaceae,Nocardiaaceae,Staphylococcus,Lactococcus,Acidovorax,Rhodococcus,Rhizobium,and Methylobacterium.Conclusion(1)Compared with healthy people,the abundance and diversity of the intestinal flora of CAG patients are reduced;the abundance of intestinal flora of CAG patients and healthy people is significantly different in each classification level.The overall trend is that the abundance of beneficial bacteria decreases.Increased the abundance of pathogenic bacteria and harmful bacteria;(2)The treatment of Jian pi hua yu jie du recipe can not only improve the clinical symptoms,but also rectify or even partially reverse the degree of atrophy and intestinal metaplasia;(3)The Jian pi hua yu jie du recipe can change the structure of the intestinal flora of CAG patients.After treatment,the structure of the intestinal flora tends to be healthy people,which shows that the abundance of beneficial bacteria is increased,and the abundance of conditional pathogenic bacteria and harmful bacteria is decreased.