| Objective Based on quantum dots labeling technology,a fast and portable immunochromatography method was established for rapid detection of brucellosis in the field.And it was also compared with the conventional brucellosis detection.Methods The whole bacterial protein of Brucella was extracted.After the concentration was determined,the whole bacterial protein was used as the labeling antigen and coating antigen.The quantum dots labeling technique and immunochromatography were used to optimize various reaction conditions and labeling conditions to prepare test strips.Different human and animal sera were tested with test strips to determine their sensitivity and specificity.Finally,compared with the Standard Agglutination Test(SAT),the Rose Bengle Plate Test(RBPT),and the colloidal gold immunochromatographic test strip,the feasibility of the test strip was analyzed.Results The whole protein of brucella has good specificity and did not cross-react with serum of different diseases.The whole bacterial protein was labeled at a concentration of 3.9 mg/ml,the coating concentration was 2.0 mg/ml,and the serum titer of the lowest detection sensitivity was 1:25.The optimum reaction time of the test strip is 25 °C ～ 30 °C.The stability of the test strip under normal temperature is good,and the repeatability is good,and the coefficient of variation is 4.0%.The sensitivity of the test strip was 98.53% and the specificity was93.57%.The coincidence rate with the tube agglutination reaction was 96.98%,which was in good agreement.The sensitivity and specificity of using the colloidal gold test strip and the tiger red plate agglutination test in series or in parallel are higher than the single method.Conclusions In this study,quantum dots immunochromatographic test strip was prepared and evaluated.By optimizing various conditions,it has certain advantages with SAT and colloidal gold test strip.It can be used for rapid detection and initial screening for brucellosis. |
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