| ObjectiveIn this study,consistency of GSTP1 gene Ile105Val polymorphism in peripheral blood and gastroscopic biopsy tissues were compared.To investigate the relationship between Ile105Val polymorphism of GSTP1 gene in peripheral blood and the efficacy and prognosis of patients with locally advanced esophageal squamous cell carcinoma who could not undergo complete resection after concurrent chemo-radio-otherapy.Metheods59 patients with esophageal squamous cell carcinoma confirmed by gastroscopic bio-psy were selected as the study subjects,collection of peripheral venous blood,and then detection of Ile105Val polymorphism of GSTP1 gene in patients by FISH.Given radiothe-rapy and chemotherapy,4 cycles of chemotherapy after radiotherapy,respectively,to eval-uate the curative effect,the effect of the best for the final evaluation results(CR,PR,SD,PD).Using SPSS 19.0 software to analyze the distribution frequency of genotypes in different patients accorded with the law of genetic balance.The influence factors(such as age,Gender,lesion length,differentiation degree,KPS score)byχ~2 check,and used Logistic regression to correction the factors.The time of disease progression(PFS)and total survival(OS)were counted.COX regression model was used to fit the relationship between survival distribution and influencing factors.And analyzed the relationship between GSTP1A105G genotype in peripheral blood and efficacy,progression free survi-val and total survival after concurrent radiotherapy and chemotherapy.To compare the relationship between different genotypes and shortterm efficacy of concurrent chemoradio-therapy and expected prognosis.Results1.According to the results of the study,the proportion of genotypes accorded with Hardy-Weinberg genetic balance rule,so the study group was representative(P>0.05).2.The genotypes of peripheral blood and gastroscopic biopsy tissues were highly consistent.They were homozygous Ile/Ile(A/A genotype 31 cases),heterozygous Ile/Val(A/G genotype 24 cases),homozygote Val/Val(G/G genotype 4 cases).3.Genotype and KPS score were independent factors by using chi-square to evaluate the relationship between therapeutic efficacy and clinical characteristics(genotye,sex,age,length of lesion,differentiation degree,T stage,N stage,KPS score).Genotype and KPS score were independent factors,P values were less than 0.05(0.007,0.037,respectively).4.Correcting the clinical characteristics of the patients by logistic regression analysis,GSTP1 genotype and KPS score were related to the curative effect of concurrent radiother-apy and chemotherapy for esophageal cancer(P<0.05).5.Statistical analysis of curative effect in patients with enrollment,the total disease control rate was 72.88%.The disease control rate of genotype A/A(41.94%)was signif-ycantly lower than that of genotype A/G and genotype G/G(89.29%),with P=0.007(<0.05),χ~2=7.256.6.The median survival and disease-free progression of patients with genotype(A/G)and(G/G)were significantly higher than those with genotype(A/A),and their P values were less than 0.05(0.03 and 0.004,respectively),with statistical differences.7.Cox proportional hazard model was used to analyze the relationship between influ-encing factors and PFS and OS.The results showed that GSTP1A105G genotype and KPS score were correlated with PFS,P value was less than 0.05.GSTP1A105G genotype was significantly correlated with OS(P<0.05).8.Analysis was used to fit the survival curve between GSTP-1A105G genotype and PFS and OS.The two curves were separated obviously.Conclusion1.GSTP1A105G gene polymorphism was detected by peripheral blood and gastrosco-pic biopsy.The gene polymerphism were the same.2.GSTP1A105G genotype polymorphism could be used as a screening index for patients with locally advanced esophageal squamous cell carcinoma before concurrent radiotherapy and chemotherapy.It could predict the sensitivity and prognosis of patients to concurrent radiotherapy and chemotherapy,and provide certain clinical guidance for their individualized treatment. |
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