The Study Of LncRNA TMPO-AS1 On The Role And Mechanism Of Hepatocellular Carcinoma

Purpose: Hepatocellular carcinoma(HCC)is characterized by high morbidity and mortality,which seriously impairs human health.Long noncoding RNAs(lnc RNAs)play a central role in the pathogenesis of various tumors,including hepatocellular carcinoma(HCC).TMPO antisense RNA 1(TMPO-AS1)has been reported to be associated with a variety of tumors.Nevertheless,the underlying mechanism whereby TMPO-AS1 influences HCC remains unclear.This study aimed to reveal the molecular mechanism governing the function of TMPO-AS1 in HCC.Methods:(1)q RT-PCR were utilized to messure TMPO-AS1 expression in HCC tissues and cell lines.Moreover,the effect of TMPO-AS1 on the overall survival rate of patients was analyzed through the Kaplan-Meier Plotter database;(2)Colony formation assays and cell counting kit-8(CCK8)were applied to assess proliferation in vitro;(3)transwell and wound healing assays were used for examination of metastasis;(4)Direct interaction of TMPO-AS1 and mi R-126-3P was demonstrated by the dual-luciferase reporter;(5)Protein biomarkers were detected using Western blot analysis.Results:(1)TMPO-AS1 was found to be remarkably overexpressed in HCC tissues and cells.(2)Furthermore,silencing of TMPO-AS1 not only inhibited HCC cell proliferation to a remarkably degree but also significantly reduced EMT-induced invasion.(3)TMPO-AS1 can target mi R-126-3p and activate the LRP6/β-catenin pathway by inhibiting mi R-126-3p.Conclusions:TMPO-AS1 can promote the proliferation,invasion and metastasis of HCC by sponging mi R-126-3p and activating LRP6/β-catenin pathway.Taken together,our study demonstrates a new molecular mechanism of TMPO-AS1,and provides a theoretical basis for TMPO-AS1 to become a new therapeutic target for HCC.