The Impact Of VEGF₁₆₅ On Neural Functional And Vascular Remodeling In Rat With Brain Hemorrhage

Objective:To explore the impact of VEGF₁₆₅ on neural function and the vessels around the hematoma in rat with cerebral hemorrhage model by injecting autologous caudal artery.Methods:60 male SD rats were randomly divided into sham-operated group,control group,VEGF₁₆₅ group.There were 24 SD rats in control group and VEGF₁₆₅ group,There were 12 SD rats in sham-operated group.Autologous tail arterial blood 50μl injection the caudate nucleus to make cerebral hemorrhage model.The sham-operated groupwas same as the rest two groups but injection blood.After cerebral hemorrhage model made in rats,adopt the NSS scores,the balance beam walking experiments and forelimbs placed experiment of judgment nerve function change.at 24 h,VEGF₁₆₅ group was given 10 μl,the concentration is 0.025μg/μl into lateral ventricle.control group was given the same amount of saline injections of lateral ventricle,sham-operated group is not given intervention.Every group was divided into four subgroups,each subgroup had 6 rats in control group and VEGF₁₆₅ group,sham-operated group only 3 rats in every subgroup.In 1 d,2 d,5 d,7 d,10 d gives the evaltation of neural the put to death in the rats and get the brain tissue.Different time of brain tissue detect CD34 protein expression through immunohistochemical staining and wesern-blot,adopting qPCR detect m RNA expression of MAP2.Results:In 2d,5d,7d,10 d control group NSS scores were 2.96±0.75,2.94±0.64,2.33±0.49,1.83±0.75;VEGF₁₆₅ group were 2.25±0.79,1.33±0.49,1.00±0.43,0.50±0.55,compared two groups of different time（P < 0.05,the difference was statistically significant）.The balance beam walking test results indicate the control group compared with VEGF₁₆₅ group at different time points（P > 0.05,there was no statistically significant difference）.Fore placement test result is shown in 2 d,5 d,7 d,10 d control scores were58.13±13.73,52.22±13.09,54.17±14.43,56.67±16.33;VEGF₁₆₅ group were 70.83±14.72,78.89±11.83,76.67±22.29,90.00±10.95,compared two groups of different time（P < 0.05,the difference was statistically significant）.HE staining results showed that compared to the 10 d and 5d the blood vessels in VEGF₁₆₅ group were better than control group.Immunohistochemical staining results suggest in 2 d,5 d,7 d,10 d the number of cells expressd CD34 is 438±45,569±105,732±90,903±103in control group;the number of cells expressd CD34 is 1506±179、1607±180、1725±92、2186±212 in VEGF₁₆₅ group,compared two groups of different time（P < 0.05,the difference was statistically significant）.According to the Western blot results in 2 d,5 d,7 d,10 d control group compared with VEGF₁₆₅,the relative expression of CD34 was lower than that in group VEGF₁₆₅.qPCR results in 2 d,5 d,7 d,10 d the MAPA relative expression were 0.10±0.27,0.07±0.00,0.32±0.10,0.59±0.19 in control group;VEGF₁₆₅ group were 0.65±0.15,1.34±0.93,2.09±0.60,2.29±0.77;At different times compared the two groups（P < 0.05,the difference was statistically significant）.Conclusions: In early cerebral hemorrhage application of VEGF₁₆₅ is beneficial to the recovery of neural function,to improve the reaction ability after hemorrhage in rats.Cerebral hemorrhage early VEGF₁₆₅ could promote the regeneration of the neural dendrites,can promote the regeneration of the cerebral hemorrhage haematoma around blood vessels.