| Objectives Prepare bioactive glass solution(BAG),and use the anti-caries experiment of isolated teeth to explore the effect of different concentrations of bioactive glass solution on the remineralization of enamel after interproximal enamel reduction(IER).Compare the remineralization effects of different remineralizing agents after deglazing the adjacent surfaces: bioactive glass,fluorine protective paint,and casein phosphopeptide-orphous calcium phosphate(CPP-ACP).Provide a theoretical basis for the prevention of dental caries after removing the glaze on the adjacent surface of clinical orthodontics.Methods Experiment one: 30 healthy isolated teeth were selected,and the adjacent surfaces were deglazed to create a model.60 specimens of the adjacent surfaces were obtained.They were randomly divided into: blank group,2% BAG group,4% BAG group and 6% BAG group.There are 15 samples in each of the 4 groups.The samples of each group were treated with deionized water and different concentrations of BAG solution,5min/time,2 times/d,30 days p H cycle,to achieve caries in vitro.Experiment two: 30 healthy isolated teeth were selected,and 60 specimens were obtained by deglazing the adjacent surfaces.They were randomly divided into 4 groups,each with 15 samples,and different mineralizers were used for remineralization treatment: blank control group: Deionized water treatment,5min/time,2 times/d;Bioactive glass group: Bioactive glass solution treatment to remove glaze,5min/time,2 times/d;Fluorine protective paint group: Fluorine protective paint for deglazing Treated only once;the mouthguard group: the mouthguard was treated to remove the glaze,5min/time,2 times/d;each group was treated with cardiopulmonary bypass for 30 days to cause caries.Experiment one and experiment two both use a microhardness tester to measure the hardness of the enamel surface(MH),scanning electron microscope(SEM)to observe the microscopic morphological characterization of the enamel surface;energy dispersive X-ray(EDX).Analyze the elements and content of the enamel surface.Results Experiment one: 1)After the experiment,the microhardness of enamel was significantly lower than before the experiment,and the difference was statistically significant(P<0.05).After the experiment,the microhardness value was the largest and the hardness difference was the smallest in the 4% BAG group.Compared with other groups,the difference was significant(P<0.05).2)Observation under the electron microscope after the experiment: the enamel surface of the blank group is extremely rough,the surface is uneven,and there are a large number of cracks caused by demineralization;the enamel surface of each group of BAG can be seen to be covered with different degrees of minerals on the surface.The mineralization deposited on the enamel surface of the 2% BAG group is mostly flakes,with uneven deposition,and obvious scratches left by the adjacent surface deglazing are still visible;the 4% BAG group has thicker mineral deposits and the adjacent surface is deglazed The scratches afterwards are significantly reduced,and the minerals are more evenly covered on the deglazed surface and are relatively flat;the enamel surface mineralization of the 6% BAG group is agglomerated and large in volume,nodules,and the deposits are obvious Visible cracks,scratches,and uneven mineralization deposits.3)The energy spectrum analysis results show that the main elements of the blank group are Ca,P,O,and the main elements of each group of BAG are Ca,P,O,Si,and Na.The calciumphosphorus ratio was highest in the 6%BAG group,followed by the 4%BAG group.There were differences in the calcium-phosphorus ratio between the groups,and the difference was statistically significant(P<0.05).Experiment two: 1)After the experiment,the microhardness of the tooth enamel was lower than before the experiment,and the difference was significant(P<0.05).After the experiment,comparing the microhardness values of each group shows that: BAG group and fluorine protective paint group The microhardness value was the highest,and the difference was statistically significant(P<0.05).2)Scanning electron microscope observation: the enamel surface of the blank group is uneven and there are a lot of demineralization pits.The minerals deposited on the enamel surface of the BAG group are more uniform and smooth,and the demineralization pores are significantly reduced.In the fluorine protective paint group,layers of minerals can be seen deposited on the enamel surface,and there are many obvious demineralization pores and cracks between the mineralization.The enamel surface of the tooth protector group is rough and uneven,and there are a large number of granular deposits covering the enamel surface,some are denser,some are looser,and demineralized pores can be seen between the calcified masses.3)The energy spectrum analysis results show that the main elements of the BAG group are Ca,P,O,Si,Na elements,the fluorine protective paint group is mainly Ca,P,O,F,and the main elements of the other groups are Ca,P,O.Comparing the ratio of calcium to phosphorus in the 4% BAG group with that of the mouthguard group,the results showed no difference(P>0.05),which were greater than those of the other groups,and the difference was statistically significant(P<0.05).Conclusions 1)The bioactive glass solution can effectively promote the remineralization of the adjacent surface deglazing,and the 4% solution has the best effect.2)Bioactive glass,fluorine protective lacquer,and tooth protector(CPP-ACP)all have the effect of promoting remineralization after removing the glaze on the adjacent surface.Among them,bioactive glass has the best remineralization effect.Figure8;Table7;Reference 111... |
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