The Effect On The Oral Squamous Cell Carcinoma Of Invasion And Metastasis Ability By Interferencing The Expression Of The SNAIL Gene

ObjectiveTo inhibit the expression of Snail through sh RNA and observe the changes of invasion and metastasis of oral squamous cell carcinoma.To explore the effect of Snail gene on oral squamous cell carcinoma.MethodsTo inhibit the expression of transcription factor Snail by RNA interference.The p AAV-U6-sh RNA-Snail-TUD-EGFP expression vector was constructed by molecular biological techniques.Then p AAV-U6-sh RNA-Snail-TUD-EGFP was co-transfected with Helper Free adeno-associated virus system p AAV-RC and p AAV-Helper into AAV-293 cell line.The silencing effect of this virus in Tca8113 was detected by RT-PCR.Detection of sh Snail protein expression in Snail cells by Western blot.The Snail gene was transfected into oral squamous cell carcinoma cell line Tca8113 by adenovirus packaging.The effects of Snail on migration and invasion of oral squamous cell carcinoma cells were investigated by cell scratch assay and Transwell assay.Results1.The result of Gene sequencing showed that sh RNA-Snail was successful constructed in p AAV-U6-sh RNA-Snail-TUD-EGFP vector.The expression level of green fluorescent protein in AAV-293 cells was monitored by the fluorescent microscopy.2.The results of RT-PCR and Western blot showed that the expression of sh RNA-snail in oral squamous cell carcinoma was significantly decreased.3.Inhibit the expression of Snail in oral squamous cell carcinoma cell line Tca8113,Transwell and cell scratch test showed that oral squamous cell carcinoma invasion and migration ability decrease.ConclusionsTo inhibit the expression of Snail in oral squamous cell carcinoma cells,and the invasion and migration ability of the cells decreased.