| Objectives To obtain the expression of Slit2 and CXCR4 protein in normal thyroid tissue,nodular goiter(NG)and papillary thyroid carcinoma(PTC),the relationship with clinicopathological characteristics,and the correlation between Slit2 and CXCR4 protein,which provides a theory for targeted therapy of PTC in accordance with.Methods Selected paraffin sections after thyroid surgery in the Glandular Surgery of Hebei General Hospital from 2018 to 2020.The medical records of all selected specimens were complete and have been confirmed by the pathological examination of the hospital.Among them,the number of cases of normal thyroid tissue(normal thyroid tissue on the non-affected side of PTC and confirmed by postoperative paraffin pathology without cancer),NG and PTC group were 16,30 and 30 respectively.The immunohistochemical staining method was used to detect the expression of Slit2 and CXCR4 protein,and the relationship between the expression of Slit2 and CXCR4 protein and the clinicopathological characteristics of PTC and the correlation between the two in PTC were analyzed.The immunohistochemical manual counting method was used to obtain the expression of Slit2 and CXCR4 protein.Finally,SPSS 22.0 software was used to statistically analyze the immunohistochemical staining results.Results 1 The experiment analyzed the data obtained immunohistochemical staining by the chi-square test.The result was that the expression of Slit2 protein in the three groups of normal thyroid tissue,NG and PTC had obviously statistical significance(χ~2=10.658,P<0.05).The expression of Slit2 in the NG was higher than that in the PTC group,and the comparison was statistically significant(P<0.0125).The expression of Slit2 in the normal thyroid group was lower than that in the PTC group,and the comparison was no statistically significant(P>0.0125).2 There was no obviously connection between Slit2protein expression and PTC patients’gender,age,tumor size,clinical stage,infiltration of thyroid capsule and number of carcinoma foci,and there was no statistically significant(P>0.05).The expression positive rate of Slit2 protein in PTC with lymph node metastasis(LNM)was 50.00%,and Slit2 protein in PTC without LNM was 91.70%,there was statistically significant(P<0.05).3 The results of the experiment the data obtained by immunohistochemical staining was that the expression of CXCR4 protein had significant statistical significance in the three groups through chi-square test(χ~2=21.896,P<0.05).The expression of CXCR4 in the normal thyroid was lower than that in the PTC group,and the comparison between the groups was statistically significant(P<0.0125).The expression of CXCR4 in the NG was lower than that in the PTC group,and the difference was statistically significant(P<0.0125).4 There was no significant relationship between the expression of CXCR4 protein and the gender,age,tumor size,infiltration of thyroid capsule,number of cancer foci,and clinical stage of PTC patients,and the difference was no statistically significant(P>0.05).The positive rate of CXCR4 protein expression in the LNM was 94.44%,and the positive rate of CXCR4 protein expression in the without LNM was 58.33%.The difference of them was obviously statistically(P<0.05).5 The spearman correlation analysis method which was used to analyze Slit2 and CXCR4 protein expression obtained by immunohistochemical staining to obtain r_svalue(correlation coefficient)and P value.In the PTC group,there was no correlation between the expression of the two protein,and the difference was no statistically significant(r_s=-0.177,P>0.05).Conclusions 1 In PTC,the expression of Slit2 protein is lower than that in NG.In PTC,the expression of CXCR4 protein is higher than that in NG and normal thyroid tissue,respectively.The Slit2 and CXCR4 protein can promote the development of PTC.2 The expression of Slit2 and CXCR4 protein are both related to LNM in PTC,indicating that have a role in promoting the metastasis of PTC.3 In the development of PTC,the expression of Slit2 and CXCR4 protein do not necessarily have an antagonistic effect.Figure2;Table7;Reference154... |
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