| Objective: Multifunctional composite chitosan /fucoidan hydrogels patch containing triamcinolone acetonide are prepared via a chemical crosslinking,which was prepared for the potential application of the treatment of oral inflammatory diseases.It has the characteristics of strong adhesion,good conformation,slight foreign body sensation and sustained release of drug.Herein,the physicochemical properties of hydrogels were evaluated.The drug release and adhesive properties of hydrogels were evaluated.Besides,the antibacterial properties,cytocompatibility and histocompatibility of hydrogels were characterized.Methods: In this study,multifunctional chitosan/fucoidan hydrogels containing triamcinolone acetonide are prepared via the epichlorohydrin(ECH)-mediated chemical crosslinking reaction.The physical structure,chemical composition,elasticity and strength of the hydrogels were tested by scanning electron microscope(SEM),fourier transforminfrared spectroscopy(FTIR),rheometer and universal testing machine,etc.Swelling ratio and water vapor transmission experiments were carried out to analyze the water absorption and permeability of the hydrogel.In vitro drug release from CFT hydrogel was detected by high performance liquid chromatography(HPLC).Meanwhile,Colony forming unit(CFU)test was used to analyze the antibacterial activity of hydrogel against Escherichia coli(E.coil)and Staphylococcus aureus(S.aureus).The cytocompatibility of hydrogel was evaluated by CCK-8 and fluorescence staining.Finally,the hydrogel was implanted into the subcutaneous tissue of rats to evaluate its histocompatibility.Results:(1)The physicochemical properties of hydrogel revealed that the hydrogels were high-water content materials.SEM showed that the pore structure was homogeneous and interconnected.It was found that the pore size of hydrogels first decreased and then increased with the addition of fucoidan and triamcinolone acetonide in turn.FTIR showed that the CFT hydrogel contained the characteristic peaks of chitosan and fucoidan,the special peaks of functional groups of triamcinolone acetonide were not exhibited,which is likely attributable to the tiny amount of triamcinolone acetonide exposed to the surface of hydrogels.In addition,the swelling ratio of hydrogel greatly increased with the addition of fucoidan,and the swelling ratio of CFT hydrogel significantly decreased with the addition of triamcinolone acetonide.However,there was no difference in water vapor transmission between components.The rheological experiments and compression tests showed that the hydrogel had good elasticity and strength.(2)The adhesion properties of hydrogels showed that the introduction of fucoidan improved the adhesive strength of hydrogels and the addition of triamcinolone acetonide did not affect the adhesive strength of hydrogels.(3)The preliminary result of drug release study illustrated CFT hydrogel has sustained release effect.(4)The antibacterial capacity of hydrogels against two common pathogenic microorganisms,i.e.,E.coil and S.aureus was performed and the result suggested that hydrogels have good antibacterial properties.(5)The results of fluorescence staining and CCK-8 indicated that hydrogels have good cytocompatibility.(6)Besides,the result of histocompatibility suggested that CFT hydrogels elicited less inflammation and promote the formation of mature and ordered collagen fibers.Conclusion: The CFT composite hydrogels were readily fabricated via the ECH chemical crosslinking.The incorporation of fucoidan and triamcinolone acetonide greatly affected the physicochemical properties of hydrogels.It was demonstrated that the CFT hydrogels possessed enhanced swelling behavior,mechanical strength,and adhesion features,and can release drugs slowly.In addition,the CFT hydrogels showed good antibacterial properties,cytocompatibility,and histocompatibility.Therefore,the developed hydrogels display great potential as oral mucosa patches for oral inflammatory diseases. |
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