Correlation Study Of MiR-146a And Ox-LDL With Preeclampsia

Objective:To study the expression of micro RNA(miR)-146 a and oxidized low-density lipoprotein(ox-LDL)in preeclampsia(PE)and their role in the pathogenesis of preeclampsia.Methods:Maternal patients admitted to the obstetrics department of Linyi Traditional Chinese Medicine Hospital between January 2018 and January 2020 were selected as the study population.30 cases each of early-onset pre-eclampsia,late-onset pre-eclampsia,preterm delivery and normal group were selected.General information on maternal data was collected,fasting venous blood was drawn before delivery,and after delivery of the placenta,tissue was collected from the maternal side of the placenta near the umbilical cord.The levels of ox-LDL in the serum of each group were measured by ELISA,the expression of miR-146 a in the serum of each group was measured by PCR,and the receptor LOX-1 of ox-LDL in the placental tissues of each group was measured by Western blot,and the differences were compared by PCR.Results:1.The general data of each group showed that there was no significant difference between the late onset PE group and the normal group in terms of age,age at menarche,gestation,delivery,gestational week of labour and BMI before delivery(P=0.980,P=0.833,P=0.494,P=0.848,P=0.353,P=0.119),which were comparable.There was no significant difference in age,age at menarche,gestation,delivery,week of delivery and BMI before delivery between the early onset PE group and the preterm delivery group(P=0.414,P=0.683,P=0.559,P=0.412,P=0.239,P=0.990),which were comparable.2.Serum ox-LDL expression,the late-onset PE group was higher than the normal group,and the difference was statistically significant(t=7.389,P<0.0001);the early-onset PE group was higher than the preterm group,and the difference was statistically significant(t =6.343,P < 0.0001);the early-onset PE group was higher than the late-onset PE group,but the comparison between the two groups was not statistically significant(P > 0.05).3.Peripheral serum miR-146 a expression,the late-onset PE group was lower than the normal group,with statistical difference(t=23.98,P<0.0001);the early-onset PE group was lower than the preterm group,and the two groups had statistical differences(t=20.59,P < 0.0001);the early-onset PE group is lower than the late-onset PE group,there is a significant difference between the two groups,which is statistically significant(t=8.92,P<0.0001).4.The expression of placenta LOX-1,the late-onset PE group was higher than the normal group,with statistical difference(t=19.86,P < 0.0001);the early-onset PE group was higher than the preterm group,the expression was statistically different(t=22.94,P < 0.0001);the early-onset PE group is higher than the late-onset PE group,there is a significant difference between the two groups,which is statistically significant(t=8.63,P < 0.0001).5.The expression of placental miR-146 a was lower in the late-onset PE group than in the normal group.There was a significant difference between the two groups and was statistically significant(t=39.87,P<0.0001);the early-onset PE group was lower than the premature group,two There is a significant difference between the two groups,which is statistically significant(t=91.31,P<0.0001);the early-onset PE group is lower than the late-onset PE group,and the comparison between the two groups is statistically significant(t=12.65,P<0.0001).6.The expression of peripheral serum miR-146 a in the early-onset PE group and the late-onset PE group was negatively correlated with the serum ox-LDL level(P<0.05);it was negatively correlated with the expression of LOX-1 in the placenta(P < 0.05).7.The expression of placental miR-146 a in the early-onset PE group and late-onset PE group was negatively correlated with the serum ox-LDL level(P<0.05),and negatively correlated with the expression of LOX-1 in the placenta(P<0.05).8.Peripheral serum ox-LDL levels in the early-onset PE group and late-onset PE group were positively correlated with the expression of LOX-1 in the placenta(P<0.05).Conclusion:The decreased expression of miR-146 a in the serum and placenta of pregnant women with preeclampsia may be involved in the pathophysiological process of preeclampsia by regulating ox-LDL and its receptor LOX-1.