Effect Of LncRNA NEAT1 Expression On Apoptosis Of Cell Model In Patients With Parkinson’s Disease Induced By MPP+

Objective:Parkinson’s disease(PD)is the second most common neurodegenerative disease.Recent studies have shown that long noncoding RNA nuclear-enriched abundant transcript 1(lnc RNA NEAT1)plays an important role in PD.We aim to investigate the effect of lnc RNA NEAT1 on apoptosis in a 1-methyl-4-phenylpyridinium ions(MPP+)-induced cellular model of PD.Methods:MPP+ at different concentrations(0,0.25,0.5,0.75,1and1.25mmol/L)were used to induce SH-SY5 Y cell to construct the PD cell model and the survival rate of SH-SY5 Y cell was tested by CCK-8 kit.LncRNA NEAT1 expression levels in PD cell model induced by MPP+ at different concentrations were detected by quantitative real-time PCR(qRT-PCR).qRT-PCR was used to detect the content of lnc RNA NEAT1 and mi R-132-3p in the control group and PD group.Western blot(WB)was used to detect the expression levels of two groups of fork head transcription factors(FOXO3a),Bim,and caspase 3;Transfect sh-NC and sh-NEAT1 into cells respectively,and measure the content of lnc RNA NEAT1 to evaluate whether lnc RNA NEAT1 was successfully knocked down..According to different treatment methods,SH-SY5 Y cells were divided into: control group,sh-NC group,sh-NC+MPP+ group and sh-NEAT1+MPP+ group,detecting the contents of mi R-132-3p in each group by qRT-PCR,and detecting the content of FOXO3 a,Bim,caspase 3 in each group by WB.Luciferase imaging was used to detect whether there is a targeting relationship between lnc RNA NEAT1 and mi R-132-3p.Results:1.The survival rate of SH-SY5 Y neuroblastoma cell treated by MPP+ was decreased distinctly with the increasing of MPP+ concentration(P<0.05);LncRNA NEAT1 expression level in PD cell model was gradually increased with the increasing of MPP+ concentration(P<0.05).1m M MPP+ has great effect on the viability of SH-SY5 Y cells,and the increased lnc RNA NEAT1 levels,which was selected for establishing an acute cellular PD model.2.qRT-PCR results showed the content of lnc RNA NEAT1 was less in SH-SY5 Y cells treated by sh-NEAT1 than cells treated by sh-NC,indicating that the lnc RNA NEAT1 in cells was successfully knocked down.3.qRT-PCR results showed that the content of mi R-132-3p was significantly decreased in sh-NC+MPP+ group compared with sh-NEAT1+MPP+,the difference was statistically significant(P<0.01).4.WB showed that compared with the control group,the expression of FOXO3 a and caspase 3 protein in the MPP+ group increased(P<0.01);the expression of FOXO3 a protein,caspase 3 protein are similar in control group compared with sh-NC(P>0.05);Compared with sh-NC+MPP+ group,the expression of FOXO3a(P<0.01),Bim(P<0.01),caspase 3(P<0.001)was less in sh-NEAT1+MPP+ group,the difference was statistically significant.5.Luciferase imaging shows that lnc RNA NEAT1 has a targeting relationship with mi R-132-3p.Conclusions:Decreasing the expression of lnc RNA NEAT1 can inhibit the apoptosis of SH-SY5 Y cells induced by MPP+ by up-regulating the expression of mi R-132-3p,which may produce a protective effect to dopaminergic neurons,providing a new strategy and potential possibility for the alleviation and treatment of PD.