Preparation Of Trametes Cinnabarinus Granules And Evaluation Of Anti-Fatigue And Hypoglycemic Effects

This experiment uses The fruiting bodies of Trametes cinnabarinusas raw material,extract the active ingredient polysaccharides from The fruiting bodies of Trametes cinnabarinusby ultrasonic-assisted extraction method and high-voltage pulsed electric field technology extraction method.Explored the technology of Trametes cinnabarinus granules,and establish quality standards;The anti-fatigue and hypoglycemic pharmacological activities of Trametes cinnabarinus were investigated.Using single factor test and orthogonal test method,the crude polysaccharides in The fruiting bodies of Trametes cinnabarinuswere extracted by ultrasonic-assisted extraction method and high-voltage pulsed electric field technology extraction method,and the process was optimized.The best extraction method of ultrasonic assisted extraction method is: the liquid-to-material ratio is 65:1,the temperature is 55°C,the initial p H value is 9,the time is 45 min,the yield of crude polysaccharide is 1.41%;the high-voltage pulsed electric field technology is the best extraction method.The method is as follows: the electric field intensity is 15 kv,the liquid-to-material ratio is65:1,the initial p H value is 9,the pulse width is 390,the yield of crude polysaccharide from Trametes versicolor fruit body is 2.04%,and the purity of crude polysaccharide is 46.02%.Compared with the ultrasonic-assisted extraction method,it has increased by 28.43%,and the purity of polysaccharide can reach 46.02%.Use the wet granulation process to determine the formula and molding process of Trametes cinnabarinus Granules as: wet granulation,the wetting agent uses the wet granulation process,determine the formula and molding process of Trametes cinnabarinus Granules: wet granulation,the wetting agent is 80% ethanol,crude polysaccharide : Dextrin: Mannitol: Stevia =1:2.4:0.6:0.03,dried at 60°C.According to the requirements of the 2015 edition of the "Chinese Pharmacopoeia" granular preparations,the Trametes cinnabarinus Granules inspection particle size and other items of different batches are in compliance with the pharmacopoeia.Anti-fatigue pharmacological tests show that Trametes cinnabarinus Granules have anti-fatigue pharmacological activity.The exercise time of the three different dosage groups of Trametes cinnabarinus Granules has been significantly increased,which can improve oxidative stress and relieve fatigue;Trametes cinnabarinus Granules body crude polysaccharide It can significantly increase the content of liver glycogen and muscle glycogen,and can effectively remove urea nitrogen and blood lactic acid in the body,accelerate the removal rate of metabolites in the body,delay fatigue,and improve tolerance.Trametes cinnabarinus Granules can significantly increase the content of liver glycogen and muscle glycogen in diabetic mice,indicating that Trametes cinnabarinus Granules can promote the conversion of glucose into glycogen,and have a hypoglycemic effect on diabetic mice,but for normal mice The blood sugar has no obvious effect.All the doses of Trametes cinnabarinus Granules decreased the blood sugar level of diabetic mice 4 weeks after administration.From the analysis of pharmacological test results,the mice’s own superoxide dismutase activity increased,and the malondialdehyde of membrane lipid peroxidation decreased.The pancreaticβ-cells reduce the damage due to the reduction of oxygen free radicals and lower blood sugar.A series of experimental studies have shown that Trametes cinnabarinus Granules have anti-fatigue and hypoglycemic pharmacological activities.The preparation process is convenient and easy to operate,which has made in-depth exploration for the in-depth research and development of Trametes cinnabarinus.