To Explore The Mechanism Of Chuanxiong In The Treatment Of Vitreous Hemorrhage Based On Network Pharmacology

Purpose: To investigate the synergistic mechanism of chuanxiong in the treatment of Vitreous hemorrhage(VH).Method: The constituents of Ligusticum chuanxiong were searched from TCMSP database as potential active constituents.The SDF structure of the above components was obtained by using Pub Chem database,and then imported into Swiss Target Prediction database.The targets with a Prediction score greater than 0 were selected as drug targets.OMIM,DISGENET and GENECARDS databases were used to retrieve "Vitreous hemorrhage" as the keyword to obtain disease action targets.The STRING database and Cytoscape 3.7.2 software were used to construct the protein-protein interaction(PPI)Network map and the "drug-active component-disease-target protein" interaction Network.The main active components of Ligusticum chuanxiong were analyzed using the Network Analyzer function.Finally,the gene ontology(GO)biological process and KEGG metabolic pathway enrichment analysis were performed on the potential therapeutic targets.Results: Seven potential active components,involving 349 proteins,were obtained by screening the active components of Ligusticum chuanxiong.There are 1500 targets for VH,90 of which are potential targets for the treatment of VH by Ligusticum Chuanxiong.There are 90 nodes in the "drug-active component-disease-target protein" interaction network,and the core target genes include EGFR、SRC、CASP3、CCND1、STAT3,etc.Through GO and KEGG enrichment analysis,125 signaling pathways related to important components of VH were screened out.Conclusion: In the process of treating VH,the potential molecular mechanism of Ligusticum Chuanxiong is related to some biological functions such as anti-angiogenesis,related regulation of apoptosis,oxidative stress response and anti-inflammatory response.and EGFR、SRC、CASP3、CCND1 and STAT3 may be the core target genes.