The Experimental Study Of The Dermal Mesenchymal Stem Cells Effect On Adhesion,Migration And Integrin Expression Of Endothelial Cells In Psoriasis

Objective: Psoriasis is a chronic,recurrent,inflammatory skin disorder,resulting from a combination of genetic and environmental factors.The disease is characterized by the hyperplasia of epidermis,inflammatory cell infiltration into dermis and epidermis,dilatation of dermal capillaries and angiogenesis.The dilatation of dermal capillaries and angiogenesis played distinct and important roles in psoriasis.And these histological changes occur in early stage and disappear finally in the local lesions of psoriatic patients.Our group has successfully isolated DMSCs from skin in psoriatic patients.We found that some proteins in psoriatic DMSCs,for instance,VEGF,epidermal growth factor-like repeats and discoidin I-like domains 3(EDIL3),platelet/endothelial cell adhesion molecule 1(PECAM-1),extracellular matrix protein gene 1(ECM-1),angiomotin(AMOT),insulin-like growth factor-binding protein 5(IGFBP5),which are related to angiogenesis,proliferation and differentiation of ECs,were expressed abnormally at gene and/or protein levels.Therefore,we speculated that DMSCs may affect adhesion and migration of human umbilical vein endothelial cells(HUVECs)in local microenvironment.Simultaneously,as important receptors,integrins(αvβ3,αvβ5 and α5β1)might be involved.Methods: The present study was aimed to evaluate whether psoriatic DMSCs could affect adhesion and migration of ECs through neovascularization-related integrins in psoriasis.Human DMSCs,collected from psoriasis lesions and healthy skin respectively,were co-cultured with human umbilical vein endothelial cells(HUVECs).The expression levels of three integrins,i.e.,αvβ3,αvβ5 and α5β1 in HUVECs were tested by quantitative real time polymerase chain reaction(q PCR)and western blot.The adhesion and migration of HUVECs were detected by adhesion assay and migration assay.Results: The results showed that in psoriasis group,the expression of αVβ3 and α5β1of HUVECs markedly increased 2.50-fold and 3.71-fold in mRNA levels,and significantly increased 1.63-fold and 1.92-fold in protein levels,comparing to healthy control group(all P<0.05).But β5 was not significantly different between the two groups(P>0.05).In addition,compared with control,psoriatic DMSCs promoted HUVECs adhesion by 1.62-fold and migration by 2.91-fold(all P<0.05).Conclusion:In conclusion,our study demonstrated that psoriatic DMSCs promoted cell-cell adhesion and migration of HUVECs,and this process was probably mediated by integrin αvβ3 and α5β1.