Experimental Study On HER2 Targeted And HSV-TK Gene-loaded Nanobubbles Combined With UTND Technology To Induce Apoptosis Of Gastric Cancer Cells

Objective: To explore the in vitro targeting and nucleus Localization effect of targeted HER2 AGM-CBA and HSV-TK gene-loaded perfluorohexane nanobubble(t HAHT NB),and the effect of t HAHT NB on the proliferation inhibition of gastric cancer cells and the induction of tumor cell apoptosis under the ultrasound-mediated nanobubble destruction technology.Methods: Using laser confocal microscopy to detect the combination of t HAHT NB with HER2-positive gastric cancer cells and HER2-negative gastric cancer cells,using Image J software for semi-quantitative analysis;using laser confocal microscopy to detect the nuclear localization effect of t HAHT NB on HER2-positive gastric cancer cells;MTT method is used to observe its effect on cell activity.Annexin V monitor its induction of apoptosis: the experiment is divided into t HAHT NB + LFUS group(t HAHT NB + low frequency ultrasound irradiation group),t HAHT NB group,t HER2 NB group(Targeted HER2 perfluorohexane nanobubbles group),NB group(blank perfluorohexane nanobubbles group),LFUS group(low frequency ultrasound irradiation group),normal cell group and black group.Results: Confocal laser The microscope showed that compared with the MGC803 group,the NCI-N87 group had obvious red fluorescence signals around the cells;semi-quantitative analysis showed that compared with the MGC803 group,the cumulative fluorescence density of the NCI-N87 group increased,and it was positively correlated with the number of cells(r=0.9005,P < 0.01);nuclear localization experiments showed that there was obvious red fluorescence around the nucleus in the NCI-N87 group.MTT method and Annexin V detection showed that compared with the t HAHT NB group,t HER2 NB,LFUS group,and NB group,the tumor cell growth activity of the t HAHT NB+LFUS group is significantly reduced,and the apoptosis rate is significantly increased;the difference is statistically significant(MTT group: t = 11.48,18.09,18.55,20.25,P <0.01;Annexin V group: t=18.00,25.74,25.53,23.23,P <0.01).Conclusion: t HAHT NB can target and identify HER2-positive gastric cancer cells,t HAHT NB combined with UTND technology can effectively improve the gene transfection rate and possess nuclear localization effect.It can significantly inhibit the proliferation of HER2-positive gastric cancer cells and induce their apoptosis,which is a non-invasive factor for gastric cancer.Invasive treatment provides new ideas.