Experimental Study On The Effect Of Different Glucose On The Uptake Of ¹⁸F-FDG In LUAD

Objective:To explore the effect of different glucose concentrations on the uptake of¹⁸F-fluorodeoxyglucose(¹⁸F-FDG)and the expression of glucose transport protein-1（Glut-1）and glucose transport protein-3（Glut-3）in lung adenocarcinoma（LUAD）.Methods:LUAD cell line A549 cells,H1299 cells and normal bronchial epithelial cell line HBE cells were cultured in DMEM medium with glucose of 3.9,5.0,6.1,8.3 and11.1 mmol/L respectively for 24 h.Then 3.7×10⁴ Bq ¹⁸F-FDG was added into each group andγcounter was used to measure the radioactivity count 1 h later.Western blot was used to examine the expression of Glut-1 and Glut-3 in A549.One-way analysis of variance and Bonferroni method were used for data analysis.The correlation was analyzed by Pearson correlation analysis.Results:The ¹⁸F-FDG uptake rates in A549 in 3.9,5.0,6.1,8.3 and 11.1 mmol/L groups were（4.89±0.83）%,（4.07±0.23）%,（3.66±0.29）%,（3.34±0.16）%and（3.29±0.24）%.respectively（F=7.05,P<0.01）.After multiple comparison,compared with 3.9mmol/L group,the ¹⁸F-FDG uptake rate in 8.3 and 11.1mmol/L groups was reduced and the differences were statistically significant（both P<0.05）,while there was no statistical difference between the other groups（all P>0.05）.The ¹⁸F-FDG uptake rates in H1299 were（5.02±0.83）%、（3.69±0.71）%、（3.29±0.23）%、（3.05±0.24）%、（2.59±0.82）%,respectively（F=6.46,P<0.01）.After multiple comparison,compared with 3.9mmol/L group,the ¹⁸F-FDG uptake rate in 8.3 and 11.1mmol/L groups was reduced and the differences were statistically significant（both P<0.05）,while there was no statistical difference between the other groups（all P>0.05）.The ¹⁸F-FDG uptake rates in H1299 were（2.65±1.32）%、（1.80±0.80）%、（1.47±0.74）%、（0.99±0.39）%、（0.60±0.17）%,and there was no significant differences statistically between the groups（P>0.05）.The relative expressions of Glut-1 and Glut-3 in A549 were 1.17±0.10,1.00±0.00,0.84±0.07,0.70±0.18,0.61±0.16,and 1.14±0.05,1.00±0.00,0.86±0.12,0.71±0.05,0.40±0.06,respectively（F value:10.29 and 51.94,all P<0.01）.Moreover,the ¹⁸F-FDG uptake rate was positive strong correlated with the expression of Glut-1and Glut-3（R value:0.775 and 0.744,all P<0.05）in A549.Conclusion:When the glucose fluctuates within 3.9～11.1mmol/L,the change of glucose will affect the ¹⁸F-FDG uptake rate in A549 cells and H1299 cells,while there was little effect on HBE cells.With the increase of glucose,the uptake rate decreases continuously and when the glucose changes greatly,the ¹⁸F-FDG uptake rate in A549cells and H1299 cells will be significantly affected.Glucose will significantly affect the expression of Glut-1 and Glut-3 in A549 cells,and the ¹⁸F-FDG uptake rate is positive strong related to the expression of Glut-1 and Glut-3.