Association Of H19 Polymorphisms With The Susceptibility Of Lung Cancer In Chinese Northeast Population

Purpose: Lung cancer is one of the main malignant tumors that threaten people’s health.The pathogenesis of lung cancer is complex,and its occurrence is the result of a variety of factors,including genetic factors,environmental factors,and health and economic conditions.Studies have found that lncRNA H19 single nucleotide polymorphism is related to the risk of a variety of diseases.H19 is considered as an effective biomarker for diagnosis and treatment.Therefore,the purpose of this study is to explore the relationship between lncRNA H19 rs2839698 and rs2071095 SNPs and lung cancer susceptibility,as well as rs2839698 and rs2071095 SNPs and smoking exposure in the Han population in Northeast China Interaction in the process of occurrence.Methods: All subjects included in this study were from the Han population in Northeast China,including 452 lung cancer cases and 463 cancer-free controls.The basic information of all research subjects was collected according to the uniformly formulated epidemiological questionnaire.At the same time,5ml of venous blood was collected for genotyping analysis.The two loci of all samples were genotyped separately by using Taq Man probe and Real-time PCR.All statistical analysis is done through SPSS 22.0.Use t-test to analyze the quantitative data.Chi-square test was used to analyze qualitative data and Hardy-Weinberg genetic balance.Through unconditional logistic regression analysis,the(odds ratio)OR,95% confidence interval and the corresponding P value were obtained.All analyses adjusted for age,gender,and smoking exposure,and obtained adjusted OR and P value.For the evaluation of the interaction between smoking exposure and single nucleotide polymorphisms,cross-sectional analysis was used.Further we use the additive model to calculate indicators reflecting the interaction,including excess relative risk and relative index.Results: The results of this study indicate that lncRNA H19(rs2839698 and rs2071095)SNPs may not be associated with lung cancer susceptibility.First,the gene frequency distributions of rs2839698 and rs2071095 in the control group are in line with the Hardy-Weinberg genetic balance.Secondly,in the overall analysis,there is no correlation between the SNPs of rs2839698 and rs2071095 and the risk of lung cancer or non-small cell lung cancer.After grouping and stratified analysis according to the average age,in the subgroup older than 60 years,for rs2839698,individuals with AG genotype have a lower risk of lung cancer than individuals with GG genotype(OR=0.525,95%CI=0.350-0.787,P=0.002).For rs2071095,individuals with AC genotype have a lower risk of lung cancer than individuals with CC genotype(OR=0.530,95%CI=0.354-0.792,P=0.002).No statistically significant results have been found in the stratified analysis of gender and smoking exposure.The interaction of lncRNA H19(rs2839698 and rs2071095)SNPs and smoking exposure on lung cancer susceptibility has not been found yet.Conclusion: Our results indicate that there may be no correlation between lncRNA H19rs2839698 and rs2071095 SNPs and lung cancer susceptibility.However,in people older than 60 years old,individuals with AG genotype rs2839698 and individuals with AC genotype rs2071095 are less susceptible to lung cancer.